Liubinskaia M M, Subbotina Iu L, Chernokhvostova E V
Zh Mikrobiol Epidemiol Immunobiol. 1984 Nov(11):101-5.
Mice received S. sonnei O-antigen at various concentrations (0.01-20,000 micrograms/ml) in drinking water. Systemic immunological memory, induced by feeding with O-antigen, was manifested by secondary immune response to parenteral boosting with homologous O-antigen or ribosomal vaccine. A pronounced priming effect was also produced by O-antigen at concentrations as low as 0.01 micrograms/ml after courses of feeding as short as 1-3 days. Even high doses of the antigen had no tolerogenic activity. The state of immunological memory was formed at least 12 days after the first feeding and lasted for a long period (at least 4 months after the last feeding). The specificity of immunological memory was proved in experiments with heterologous O-antigen (Salmonella typhimurium): the insignificant stimulating action of this antigen was revealed only when high concentrations of the antigen (1000 micrograms/ml) were used for feeding.
小鼠饮用含有不同浓度（0.01 - 20,000微克/毫升）宋内志贺氏菌O抗原的水。通过喂食O抗原诱导的全身免疫记忆表现为对用同源O抗原或核糖体疫苗进行肠胃外加强免疫的二次免疫反应。在短至1 - 3天的喂食疗程后，低至0.01微克/毫升的O抗原也产生了明显的启动效应。即使是高剂量的抗原也没有致耐受性活性。免疫记忆状态在首次喂食后至少12天形成，并持续很长一段时间（最后一次喂食后至少4个月）。在使用异源O抗原（鼠伤寒沙门氏菌）的实验中证明了免疫记忆的特异性：仅当使用高浓度抗原（1000微克/毫升）进行喂食时，才发现该抗原具有微不足道的刺激作用。
