Marone G, Tamburini M, Giudizi M G, Biagiotti R, Almerigogna F, Romagnani S
Agents Actions. 1985 Jul;16(5):359-62. doi: 10.1007/BF01982873.
We investigated the capacity of Staphylococcus aureus Cowan I (Cowan Staph A+) and Staphylococcus aureus Wood 46 (Wood Staph A-) to induce histamine release from human basophils in vitro. Cowan Staph A+ (3 X 10(6) to 3 X 10(8)/ml), which synthesizes protein A (Staph A), stimulated the release of histamine from basophils, whereas Wood Staph A- (3 X 10(6) to 3 X 10(9)/ml), which does not synthesize Staph A, did not induce histamine secretion. Soluble Staph A (10(-3) to 10 micrograms/ml) also induced histamine secretion from human basophils, Hyperiodination of Staph A, which destroys over 90% of the original Fc reactivity without altering the Fab binding site, did not alter this protein's ability to induce histamine release. The stimulating effect of Staph A was suppressed by preincubation with human polyclonal IgG and a human monoclonal IgM, which have F(ab')-Staph A reactivity. In contrast, rabbit IgG and a human monoclonal IgM possessing only Fc-Staph A reactivity did not inhibit Staph A activity. Preincubation with Staph A or Cowan Staph A+ resulted in complete cross-desensitization to a subsequent challenge with homologous and heterologous stimuli. These results indicate that Staph A and Cowan Staph A+ activate human basophils by interacting with the F(ab')2 region of IgE and/or IgG present on the cell surface.
我们研究了金黄色葡萄球菌考恩I型（考恩葡萄球菌A+）和金黄色葡萄球菌伍德46型（伍德葡萄球菌A-）在体外诱导人嗜碱性粒细胞释放组胺的能力。能合成蛋白A（葡萄球菌A）的考恩葡萄球菌A+（3×10⁶至3×10⁸/ml）刺激嗜碱性粒细胞释放组胺，而不合成葡萄球菌A的伍德葡萄球菌A-（3×10⁶至3×10⁹/ml）则不诱导组胺分泌。可溶性葡萄球菌A（10⁻³至10微克/ml）也诱导人嗜碱性粒细胞分泌组胺。对葡萄球菌A进行高碘酸盐处理，可破坏超过90%的原始Fc反应性而不改变Fab结合位点，但这并不改变该蛋白诱导组胺释放的能力。葡萄球菌A的刺激作用可被与人多克隆IgG和人单克隆IgM预孵育所抑制，这两种抗体具有F(ab')-葡萄球菌A反应性。相反，仅具有Fc-葡萄球菌A反应性的兔IgG和人单克隆IgM并不抑制葡萄球菌A的活性。用葡萄球菌A或考恩葡萄球菌A+预孵育可导致对随后同源和异源刺激的完全交叉脱敏。这些结果表明，葡萄球菌A和考恩葡萄球菌A+通过与细胞表面存在的IgE和/或IgG的F(ab')₂区域相互作用来激活人嗜碱性粒细胞。
