葡萄球菌蛋白A对人嗜碱性粒细胞的激活作用。I. 环磷酸腺苷、花生四烯酸代谢产物、微管和微丝的作用。
Activation of human basophils by staphylococcal protein A. I. The role of cyclic AMP, arachidonic acid metabolites, microtubules and microfilaments.
作者信息
Marone G, Poto S, Petracca R, Triggiani M, de Lutio di Castelguidone E, Condorelli M
出版信息
Clin Exp Immunol. 1982 Dec;50(3):661-8.
Protein A from Staphylococcus aureus (Staph A) induces histamine secretion from human basophil leucocytes in the concentration range 10(-4) - 10 micrograms/ml. This reaction has great similarities to that of antigen or anti-IgE-induced release. It is characterized by a two stage reaction, requires extracellular calcium and is optimal at 37 degrees C. The rate of release is similar to that of IgE-mediated reactions. Histamine release induced by Staph A is inhibited by metabolic inhibitors, drugs which increase intracellular cyclic AMP levels, inhibitors of lipoxygenase pathways and a phospholipase A2 inhibitor. D2O and cytochalasin B which affect microtubules and microfilaments respectively, enhance histamine release induced by Staph A. These results suggest that Staph A-induced release is modulated by intracellular cyclic AMP, arachidonic acid metabolites, requires energy and is enhanced by the disruption of microfilaments and stabilization of microtubules.
金黄色葡萄球菌的A蛋白（葡萄球菌A蛋白）在10⁻⁴ - 10微克/毫升的浓度范围内可诱导人嗜碱性白细胞分泌组胺。该反应与抗原或抗IgE诱导的释放反应极为相似。其特点是有两个阶段的反应，需要细胞外钙，且在37℃时最为适宜。释放速率与IgE介导的反应相似。葡萄球菌A蛋白诱导的组胺释放受到代谢抑制剂、增加细胞内环磷酸腺苷水平的药物、脂氧合酶途径抑制剂和磷脂酶A2抑制剂的抑制。分别影响微管和微丝的重水和细胞松弛素B可增强葡萄球菌A蛋白诱导的组胺释放。这些结果表明，葡萄球菌A蛋白诱导的释放受细胞内环磷酸腺苷、花生四烯酸代谢物调节，需要能量，且微丝的破坏和微管的稳定可增强该释放。
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