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New matrices for the purification of pectinases by affinity chromatography.

作者信息

Lobarzewski J, Fiedurek J, Ginalska G, Wolski T

出版信息

Biochem Biophys Res Commun. 1985 Sep 16;131(2):666-74. doi: 10.1016/0006-291x(85)91289-6.

DOI:10.1016/0006-291x(85)91289-6
PMID:2413847
Abstract

Polygalacturonic acid was used as a ligand in the affinity technique for pectinases purification from the filtrate of Aspergillus niger 71 culture. For this purpose four matrices were examined, namely, alkylamine controlled porous glass (CPG), alkylamine silica gel as well as keratin or polyamide coated silica gel. Good results of pectinase purification was obtained on silanized CPG or keratin coated silica gel supports.

摘要

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