Roos P H, Hartman H J, Schlepper-Schäfer J, Kolb H, Kolb-Bachofen V
Biochim Biophys Acta. 1985 Oct 30;847(1):115-21. doi: 10.1016/0167-4889(85)90161-2.
We have isolated a galactose-specific receptor protein from rat liver macrophages by three techniques, all using EDTA extraction and subsequent affinity chromatography. The purified receptor has an apparent molecular mass of 30 kDa and exhibits hemagglutinating activity. Monospecific receptor-antisera produce one precipitation line with the macrophage receptor in Ouchterlony double diffusion but show no cross-reaction with the hepatocyte receptor. Sinusoidal cells, but not hepatocytes, are stained with monoclonal antibodies to the macrophage receptor, whereas anti-hepatocyte receptor antibodies stain hepatocyte surfaces but not sinusoidal cells. We conclude that the galactose-specific receptor from liver macrophages is structurally different from the hepatocyte receptor, although the two lectins share a similar binding specificity.
我们通过三种技术从大鼠肝脏巨噬细胞中分离出一种半乳糖特异性受体蛋白，所有技术均使用乙二胺四乙酸（EDTA）提取及后续亲和层析法。纯化后的受体表观分子量为30 kDa，并具有血凝活性。单特异性受体抗血清在双向免疫扩散试验中与巨噬细胞受体产生一条沉淀线，但与肝细胞受体无交叉反应。针对巨噬细胞受体的单克隆抗体可使窦状隙细胞染色，而肝细胞不着色，而抗肝细胞受体抗体则使肝细胞表面染色，窦状隙细胞不着色。我们得出结论，肝脏巨噬细胞的半乳糖特异性受体在结构上与肝细胞受体不同，尽管这两种凝集素具有相似的结合特异性。
