Mitranic M, Sturgess J M, Moscarello M A
Biochim Biophys Acta. 1976 Aug 16;443(2):190-7. doi: 10.1016/0005-2736(76)90502-2.
The effect of temperature on the activity of galactosyl- and sialyltransferases of rat liver Golgi membranes and the galactosyltransferase of serum has been studied. Arrhenius plots for three enzymes were different. Sharp breaks in the curves, indicative of phase transitions were observed for sialyltransferase (28 degree C) of Golgi and galactosyltransferase (34 degree C) of serum but not for galactosyltransferase of Golgi. The activation energy was greater above the break (above 28 degree C) than below for sialyltransferase of Golgi; The activation energy was lower (above 34 degree C) for galactosyltransferase of serum than below. Electron microscopic freeze replicas showed a patchy distribution of particles which increased as the temperature was raised accompanied by smooth areas. This was interpreted as representing lateral phase separation of the membrane components.
研究了温度对大鼠肝脏高尔基膜的半乳糖基转移酶和唾液酸转移酶以及血清半乳糖基转移酶活性的影响。三种酶的阿累尼乌斯曲线不同。在高尔基唾液酸转移酶（28℃）和血清半乳糖基转移酶（34℃）的曲线中观察到明显的断点，表明存在相变，但高尔基半乳糖基转移酶的曲线未观察到。高尔基唾液酸转移酶在断点以上（28℃以上）的活化能大于断点以下；血清半乳糖基转移酶在34℃以上的活化能低于34℃以下。电子显微镜冷冻复制品显示颗粒呈斑块状分布，随着温度升高颗粒增加，同时伴有平滑区域。这被解释为代表膜成分的侧向相分离。
