Department of Microbiology and Virology, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland.
J Environ Manage. 2013 Dec 15;131:55-65. doi: 10.1016/j.jenvman.2013.09.041. Epub 2013 Oct 18.
This is the first report describing the effect of imidacloprid applied at field rate (FR, 1 mg/kg of soil) and 10 times the FR (10*FR, 10 mg/kg of soil) on the structural, genetic and physiological diversity of soil bacterial community as determined by the phospholipid fatty acid (PLFA), the denaturing gradient gel electrophoresis (DGGE), and the community level physiological profile (CLPP) approaches. PLFA profiles showed that imidacloprid significantly shifted the microbial community structure and decreased the biomass of the total, bacterial and fungal PLFAs, however, this effect was transient at the FR dosage. The alterations in DGGE patterns caused by imidacloprid application, confirmed considerable changes in the overall richness and diversity of dominant bacteria. Although, as a result of imidacloprid application, the metabolic activity of microbial communities was generally lower, the richness and functional biodiversity of the soil microbial community were not negatively affected. In general, the analysis of the variance indicated that the measured parameters were significantly affected by treatment and the incubation time, however, the incubation time effect explained most of the observed variance. Imidacloprid degradation and the appearance of some new bands in DGGE profiles suggest the evolution of bacteria capable of degrading imidacloprid among indigenous microflora.
这是首个描述吡虫啉以田间推荐剂量（FR，1mg/kg 土壤）和 10 倍田间推荐剂量（10*FR，10mg/kg 土壤）施用于土壤后对土壤细菌群落结构、遗传和生理多样性影响的报告。通过磷脂脂肪酸（PLFA）、变性梯度凝胶电泳（DGGE）和群落水平生理图谱（CLPP）方法分析，PLFA 图谱表明吡虫啉显著改变了微生物群落结构，降低了总 PLFA、细菌和真菌 PLFA 的生物量，但在 FR 剂量下，这种影响是短暂的。吡虫啉处理引起的 DGGE 图谱的改变证实了优势细菌的总体丰富度和多样性发生了相当大的变化。尽管吡虫啉处理后微生物群落的代谢活性通常较低，但土壤微生物群落的丰富度和功能多样性并未受到负面影响。总的来说，方差分析表明，处理和孵育时间显著影响了所测量的参数，但孵育时间的影响解释了大部分观察到的方差。吡虫啉的降解和 DGGE 图谱中出现的一些新条带表明，土著微生物群落中出现了能够降解吡虫啉的细菌的进化。
