Chong Shau Poh, Lai Tom, Zhou Yifeng, Tang Shuo
Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Biomed Opt Express. 2013 Aug 8;4(9):1584-94. doi: 10.1364/BOE.4.001584. eCollection 2013.
Multi-scale multimodal microscopy is a very useful technique by providing multiple imaging contrasts with adjustable field of views and spatial resolutions. Here, we present a tri-modal microscope combining multiphoton microscopy (MPM), optical coherence microscopy (OCM) and optical coherence tomography (OCT) for subsurface visualization of biological tissues. The advantages of the tri-modal system are demonstrated on various biological samples. It enables the visualization of multiple intrinsic contrasts including scattering, two-photon excitation fluorescence (TPEF), and second harmonic generation (SHG). It also enables a rapid scanning over a large tissue area and a high resolution zoom-in for cellular-level structures on regions of interest. The tri-modal microscope can be important for label-free imaging to obtain a sufficient set of parameters for reliable sample analysis.
多尺度多模态显微镜是一项非常有用的技术，它能提供具有可调节视野和空间分辨率的多种成像对比度。在此，我们展示了一种结合多光子显微镜（MPM）、光学相干显微镜（OCM）和光学相干断层扫描（OCT）的三模态显微镜，用于生物组织的亚表面可视化。该三模态系统的优势在各种生物样本上得到了证明。它能够实现多种固有对比度的可视化，包括散射、双光子激发荧光（TPEF）和二次谐波产生（SHG）。它还能够在大组织区域进行快速扫描，并对感兴趣区域的细胞水平结构进行高分辨率放大观察。这种三模态显微镜对于无标记成像很重要，能够获取足够的参数集以进行可靠的样本分析。
