*Hematopathology Unit, Pathology Department, Hospital Clínic ‡Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) §Department of Anatomic Pathology, Pharmacology and Microbiology, University of Barcelona, Barcelona, Spain †Institute of Surgical Pathology, University Hospital Zurich, Zurich, Switzerland ∥Research and Development, Atlas Antibodies, Stockholm, Sweden.
Am J Surg Pathol. 2014 Jan;38(1):86-93. doi: 10.1097/PAS.0b013e3182a43996.
The diagnosis of mantle cell lymphoma (MCL) can be difficult, especially when no t(11;14) translocation and cyclin D1 overexpression can be detected. In such cases, the transcription factor SOX11 represents an important diagnostic marker, as it is expressed in most MCLs and, in particular, in all cyclin D1-negative MCLs reported so far. A reliable anti-SOX11 antibody is therefore a very useful tool for routine diagnosis. Here, we characterize the new monoclonal anti-SOX11 antibodies, suitable for Western blot assay and immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue; we tested them on a large series of primary lymphoid tumors and compared these results with those of other routinely used antibodies. Moreover, we show that IHC results depend on transcription levels of SOX11, which suggests that posttranscriptional and posttranslational modifications do not significantly affect cutoff levels for IHC detection of SOX11.
套细胞淋巴瘤（MCL）的诊断可能具有挑战性，尤其是当无法检测到 t(11;14)易位和 cyclin D1 过表达时。在这种情况下，转录因子 SOX11 是一个重要的诊断标志物，因为它在大多数 MCL 中表达，特别是在迄今为止报道的所有 cyclin D1 阴性的 MCL 中表达。因此，一种可靠的抗 SOX11 抗体是常规诊断的非常有用的工具。在这里，我们对新的单克隆抗 SOX11 抗体进行了特征描述，这些抗体适用于 Western blot 检测和福尔马林固定石蜡包埋组织的免疫组织化学（IHC）；我们在一系列原发性淋巴肿瘤上对其进行了测试，并将这些结果与其他常用抗体的结果进行了比较。此外，我们表明 IHC 结果取决于 SOX11 的转录水平，这表明转录后和翻译后修饰不会显著影响 SOX11 的 IHC 检测的截断值。
