Radovanovic Ana M, Cupara Snezana M, Popovic Suzana Lj, Tomovic Marina T, Slavkovska Violeta N, Jankovic Slobodan M
Pharmacy Department, Faculty of Medical Sciences, University of Kragujevac, Serbia.
Acta Pol Pharm. 2013 Sep-Oct;70(5):851-4.
Potentilla reptans L. belongs to the least studied of the plants from Rosaceae family, Potentilla genus. There are no data on cytotoxicity of P. reptans extracts, though traditionaly it was used as antiinflammatory and antiinfective. The aim of these studies was to investigate potential antitumor activity of aqueous extracts (rhizome and aerial parts) of P. reptans on 4T1 mouse breast cancer cell line. _Aqueous extracts of rhizome and aerial parts of P. reptans were tested for cytotoxicity by the MTT colorimetric assay on 4T1 cancer cell line in concentration range 100-800 microg/mL. Aqueous extracts of P. reptans rhizome and aerial parts show concentration dependent cytotoxic effect in the range of tested concentrations. ICE50 value of P. reptans rhizome extract was 280.51 +/- 1.16 microg/mL. IC50 value of P. reptans aerial parts extract was 310.79 +/- 1.22 microg/mL. The significant difference in cytotoxicity among tested concentrations was observed. Aqueous extracts of P. reptans rhizome and aerial parts demonstrated weak cytotoxic activity on 4T1 mouse breast cancer cell line, which is in correlation with current cytotoxicity data for aqueous herbal extracts. Rhizome extract of P. reptans has slightly higher antitumor activity than aerial parts extract. The results represent the first report on cytotoxicity for this plant and further research on human cell lines is indicated.
蛇莓隶属于蔷薇科委陵菜属中研究最少的植物。目前尚无关于蛇莓提取物细胞毒性的数据，尽管传统上它被用作抗炎和抗感染药物。这些研究的目的是调查蛇莓根茎和地上部分的水提取物对4T1小鼠乳腺癌细胞系的潜在抗肿瘤活性。通过MTT比色法在浓度范围为100 - 800μg/mL的条件下，对蛇莓根茎和地上部分的水提取物在4T1癌细胞系上进行细胞毒性测试。蛇莓根茎和地上部分的水提取物在所测试的浓度范围内呈现出浓度依赖性细胞毒性作用。蛇莓根茎提取物的IC50值为280.51±1.16μg/mL。蛇莓地上部分提取物的IC50值为310.79±1.22μg/mL。在所测试的浓度之间观察到了细胞毒性的显著差异。蛇莓根茎和地上部分的水提取物对4T1小鼠乳腺癌细胞系表现出较弱的细胞毒性活性，这与目前草药水提取物的细胞毒性数据相关。蛇莓根茎提取物的抗肿瘤活性略高于地上部分提取物。这些结果代表了关于该植物细胞毒性的首次报道，并表明需要对人类细胞系进行进一步研究。
