Takata R, Mukai T, Hori K
Nucleic Acids Res. 1985 Oct 25;13(20):7289-97. doi: 10.1093/nar/13.20.7289.
Ribosomal protein S15 and polynucleotide phosphorylase of E. coli are encoded by two adjacent genes, rpsO and pnp, respectively. Analysis of in vivo transcripts from these two genes shows that they are within the same operon (S15 operon). By correlating the 5' and 3' ends of their in vivo transcripts with the DNA sequence, we have identified several features of the operon structure. These features include a promotor upstream from rpsO, an attenuator downstream from rpsO and an RNA processing site between these two genes.
大肠杆菌的核糖体蛋白S15和多核苷酸磷酸化酶分别由两个相邻的基因rpsO和pnp编码。对这两个基因的体内转录本分析表明,它们位于同一个操纵子(S15操纵子)内。通过将它们体内转录本的5'端和3'端与DNA序列相关联,我们确定了操纵子结构的几个特征。这些特征包括rpsO上游的一个启动子、rpsO下游的一个弱化子以及这两个基因之间的一个RNA加工位点。