Jones P G, VanBogelen R A, Neidhardt F C
J Bacteriol. 1987 May;169(5):2092-5. doi: 10.1128/jb.169.5.2092-2095.1987.
When the growth temperature of an exponential culture of Escherichia coli is abruptly decreased from 37 to 10 degrees C, growth stops for several hours before a new rate of growth is established. During this growth lag the number of proteins synthesized is dramatically reduced, and at one point only about two dozen proteins are made; 13 of these are made at differential rates that are 3 to 300 times increased over the rates at 37 degrees C. The protein with the highest rate of synthesis during the lag is not detectably made at 37 degrees C. The identities of several of these cold shock proteins correlate with previous observations that indicate a block in translation initiation at low temperatures.
当大肠杆菌指数生长期培养物的生长温度从37℃突然降至10℃时，生长会停止数小时，之后才会建立新的生长速率。在这个生长延迟期，合成的蛋白质数量会大幅减少，在某一时刻，仅合成约24种蛋白质；其中13种蛋白质的合成速率与在37℃时相比增加了3至300倍。延迟期合成速率最高的蛋白质在37℃时无法检测到。其中几种冷休克蛋白的特性与之前的观察结果相符，这些观察结果表明低温会阻碍翻译起始。
