Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, PO Box 9196, One Medical Center Drive, Morgantown, WV 26506, USA; Department of Exercise Physiology, West Virginia University, Morgantown, WV 26506, USA.
Biomaterials. 2014 Jan;35(2):642-53. doi: 10.1016/j.biomaterials.2013.09.099. Epub 2013 Oct 19.
Autologous cells suffer from limited cell number and senescence during ex vivo expansion for cartilage repair. Here we found that expansion on extracellular matrix (ECM) deposited by fetal synovium-derived stem cells (SDSCs) (FE) was superior to ECM deposited by adult SDSCs (AE) in promoting cell proliferation and chondrogenic potential. Unique proteins in FE might be responsible for the rejuvenation effect of FE while advantageous proteins in AE might contribute to differentiation more than to proliferation. Compared to AE, the lower elasticity of FE yielded expanded adult SDSCs with lower elasticity which could be responsible for the enhancement of chondrogenic and adipogenic differentiation. MAPK and noncanonical Wnt signals were actively involved in ECM-mediated adult SDSC rejuvenation.
自体细胞在体外扩增进行软骨修复时，会受到细胞数量有限和衰老的限制。在这里，我们发现，在由胎儿滑膜来源的干细胞（SDSCs）（FE）分泌的细胞外基质（ECM）上进行扩增，比在由成体 SDSCs（AE）分泌的 ECM 上进行扩增，更能促进细胞增殖和软骨形成潜力。FE 中独特的蛋白质可能是 FE 具有年轻化作用的原因，而 AE 中有利的蛋白质可能更有利于分化而不是增殖。与 AE 相比，FE 的较低弹性导致扩增的成体 SDSCs 具有较低的弹性，这可能是增强软骨形成和脂肪形成分化的原因。MAPK 和非经典 Wnt 信号在 ECM 介导的成体 SDSC 年轻化中起着积极的作用。
