Li Jingting, Pei Ming
Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, Division of Exercise Physiology, and Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV, USA.
Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, PO Box 9196, One Medical Center Drive, Morgantown, WV, 26506-9196, USA.
Methods Mol Biol. 2018;1577:147-154. doi: 10.1007/7651_2017_27.
Traditional ex vivo expansion of adult stem cells yields an insufficient quantity of less potent cells. Here we describe the fabrication of decellularized matrix deposited by synovium-derived stem cells (SDSCs). This matrix could serve as a three-dimensional expansion system to rejuvenate cells for proliferation and tissue-specific differentiation potential, which could benefit cartilage regeneration. The decellularized stem cell matrix (DSCM) might be a powerful system for tissue engineering and regeneration.
传统的成体干细胞体外扩增产生的细胞数量不足且效力较低。在此，我们描述了由滑膜来源的干细胞（SDSCs）沉积的脱细胞基质的制备。这种基质可作为一种三维扩增系统，使细胞恢复活力以进行增殖和组织特异性分化，这可能有利于软骨再生。脱细胞干细胞基质（DSCM）可能是一种用于组织工程和再生的强大系统。
