一种制备去细胞化干细胞基质以促进细胞扩增和软骨再生的方案。
A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration.
作者信息
Li Jingting, Pei Ming
机构信息
Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, Division of Exercise Physiology, and Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV, USA.
Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, PO Box 9196, One Medical Center Drive, Morgantown, WV, 26506-9196, USA.
出版信息
Methods Mol Biol. 2018;1577:147-154. doi: 10.1007/7651_2017_27.
Abstract
Traditional ex vivo expansion of adult stem cells yields an insufficient quantity of less potent cells. Here we describe the fabrication of decellularized matrix deposited by synovium-derived stem cells (SDSCs). This matrix could serve as a three-dimensional expansion system to rejuvenate cells for proliferation and tissue-specific differentiation potential, which could benefit cartilage regeneration. The decellularized stem cell matrix (DSCM) might be a powerful system for tissue engineering and regeneration.
摘要
传统的成体干细胞体外扩增产生的细胞数量不足且效力较低。在此，我们描述了由滑膜来源的干细胞（SDSCs）沉积的脱细胞基质的制备。这种基质可作为一种三维扩增系统，使细胞恢复活力以进行增殖和组织特异性分化，这可能有利于软骨再生。脱细胞干细胞基质（DSCM）可能是一种用于组织工程和再生的强大系统。
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Ascorbate-dependent impact on cell-derived matrix in modulation of stiffness and rejuvenation of infrapatellar fat derived stem cells toward chondrogenesis.抗坏血酸盐对细胞衍生基质的依赖性影响，可调节髌下脂肪来源干细胞的硬度并使其向软骨形成方向年轻化。
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Tissue Eng Part A. 2013 May;19(9-10):1144-54. doi: 10.1089/ten.TEA.2012.0351. Epub 2013 Jan 15.
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Stem Cells Dev. 2013 Mar 15;22(6):889-900. doi: 10.1089/scd.2012.0495. Epub 2012 Dec 16.
7
Synovium-derived stem cells: a tissue-specific stem cell for cartilage engineering and regeneration.滑膜衍生的干细胞：一种用于软骨工程和再生的组织特异性干细胞。
Tissue Eng Part B Rev. 2012 Aug;18(4):301-11. doi: 10.1089/ten.TEB.2012.0002. Epub 2012 Apr 19.
8
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Cell senescence: a challenge in cartilage engineering and regeneration.细胞衰老：软骨工程与再生面临的挑战。
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