Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran; Department of Genetics, Qazvin University of Medical Sciences, Qazvin, Iran.
Toxicol Lett. 2014 Jan 3;224(1):108-13. doi: 10.1016/j.toxlet.2013.10.010. Epub 2013 Oct 20.
Flavonoids are present in foods such as fruits and vegetables. A relationship between the consumption of flavonoid-rich foods and prevention of human disease including neurodegenerative disorders has been demonstrated. We assessed the effect of rutin (3,3',4',5,7-pentahydroxyflavone-3-rhamnoglucoside) on the mitogen-activated protein kinase (MAPK) pathway, memory retrieval and oxidative stress in rats injected with β-amyloid (Aβ), which is implicated to have an important role in Alzheimer's disease (AD). Aβ was injected bilaterally in the deep frontal cortex of rat brain. Next, rutin and saline were injected (i.p.) for 3 weeks. In comparison to the control group, rutin significantly increased extracellular signal-regulated protein kinase 1 (ERK1), cAMP response element-binding protein (CREB) and brain-derived neurotrophic factor (BDNF) gene expression in the hippocampus of rats. Rutin (100 mg/kg) significantly increased memory retrieval compared to the control group. Malondialdehyde (MDA) level in the hippocampus of the rutin group was significantly lower than those in the control group. The content of sulfhydryl groups in the rutin group was higher than that in the control group. The findings show a possibility that rutin may have beneficial effects against neurotoxicity of Aβ on memory in rats.
类黄酮存在于水果和蔬菜等食物中。已经证明，富含类黄酮的食物的消费与包括神经退行性疾病在内的人类疾病的预防之间存在关系。我们评估了芦丁（3,3',4',5,7-五羟基黄酮-3-鼠李糖苷）对β-淀粉样蛋白（Aβ）注射大鼠的丝裂原活化蛋白激酶（MAPK）通路、记忆检索和氧化应激的影响，Aβ 被注射到大鼠大脑的深额叶皮质中。接下来，芦丁和生理盐水（ip）注射 3 周。与对照组相比，芦丁显著增加了大鼠海马中细胞外信号调节蛋白激酶 1（ERK1）、cAMP 反应元件结合蛋白（CREB）和脑源性神经营养因子（BDNF）基因的表达。与对照组相比，芦丁（100mg/kg）显著提高了记忆检索能力。芦丁组海马中的丙二醛（MDA）水平明显低于对照组。芦丁组的巯基含量高于对照组。研究结果表明，芦丁可能对大鼠 Aβ 的神经毒性对记忆具有有益的影响。
