Marakhova I I, Efimova E V, Vinogradova T A
Tsitologiia. 1987 Jan;29(1):59-65.
The cultures of Chinese hamster ovary cells (CHO-K1 clone 773) can be brought to the stationary state with most of cellular populations in G1 phase by growing continuously for 4 days up to the cultural density (10-12) X 10(4) cells/cm2. Upon introduction of fresh Eagle medium with 10% calf serum the cells progress from G1 to S phase for 7-9 hours. It is shown that within the first minutes of serum addition ouabain-sensitive rubidium influx increases, however, lithium influx, which serves a test for passive sodium pathways in the membrane, increases or does not change. No correlation was found between the rubidium influx and intracellular sodium changes, induced by serum. From comparative studies of ouabain-sensitive rubidium influx, lithium influx and intracellular sodium content it is concluded that the increase in intracellular sodium is not responsible for serum-induced Na,K-ATPase activation.
通过连续培养4天直至培养密度达到(10 - 12)×10⁴个细胞/cm²，中国仓鼠卵巢细胞（CHO - K1克隆773）的培养物可进入静止状态，此时大多数细胞群体处于G1期。加入含10%小牛血清的新鲜伊格尔培养基后，细胞在7 - 9小时内从G1期进入S期。结果表明，在添加血清的最初几分钟内，哇巴因敏感的铷流入增加，然而，作为膜上被动钠途径检测指标的锂流入增加或不变。未发现铷流入与血清诱导的细胞内钠变化之间存在相关性。通过对哇巴因敏感的铷流入、锂流入和细胞内钠含量的比较研究得出结论，细胞内钠的增加并非血清诱导的Na,K - ATP酶激活的原因。
