Rubino Corrado, Mazzarello Vittorio, Faenza Mario, Montella Andrea, Santanelli Fabio, Farace Francesco
From the Department of Medicine and Surgery, University of Salerno, Baronissi (Salerno), Italy.
Ann Plast Surg. 2015 Jun;74(6):718-21. doi: 10.1097/SAP.0b013e3182a1e5a4.
The aim of this study was to evaluate the effects on adipocyte morphology of 2 techniques of fat harvesting and of fat purification in lipofilling, considering that the number of viable healthy adipocytes is important in fat survival in recipient areas of lipofilling.
Fat harvesting was performed in 10 female patients from flanks, on one side with a 2-mm Coleman cannula and on the other side with a 3-mm Mercedes cannula. Thirty milliliter of fat tissue from each side was collected and divided into three 10 mL syringes: A, B, and C. The fat inside syringe A was left untreated, the fat in syringe B underwent simple sedimentation, and the fat inside syringe C underwent centrifugation at 3000 rpm for 3 minutes. Each fat graft specimen was processed for examination under low-vacuum scanning electron microscope. Diameter (μ) and number of adipocytes per square millimeter and number of altered adipocytes per square millimeter were evaluated. Untreated specimens harvested with the 2 different techniques were first compared, then sedimented versus centrifuged specimens harvested with the same technique were compared. Statistical analysis was performed using Wilcoxon signed rank test.
The number of adipocytes per square millimeter was statistically higher in specimens harvested with the 3-mm Mercedes cannula (P = 0.0310). The number of altered cells was statistically higher in centrifuged specimens than in sedimented ones using both methods of fat harvesting (P = 0.0080) with a 2-mm Coleman cannula and (P = 0.0050) with a 3-mm Mercedes cannula. Alterations in adipocyte morphology consisted in wrinkling of the membrane, opening of pore with leakage of oily material, reduction of cellular diameter, and total collapse of the cellular membrane.
Fat harvesting by a 3-mm cannula results in a higher number of adipocytes and centrifugation of the harvested fat results in a higher number of morphologic altered cells than sedimentation.
本研究的目的是评估脂肪填充术中两种脂肪采集技术和脂肪纯化技术对脂肪细胞形态的影响，因为在脂肪填充的受区，存活健康的脂肪细胞数量对脂肪存活至关重要。
对10名女性患者的侧腹进行脂肪采集，一侧使用2毫米的科尔曼套管，另一侧使用3毫米的梅赛德斯套管。从每侧收集30毫升脂肪组织，并分成三个10毫升的注射器：A、B和C。注射器A中的脂肪不做处理，注射器B中的脂肪进行简单沉淀，注射器C中的脂肪以3000转/分钟的速度离心3分钟。每个脂肪移植标本进行低真空扫描电子显微镜检查。评估每平方毫米脂肪细胞的直径（微米）、数量以及每平方毫米形态改变的脂肪细胞数量。首先比较用两种不同技术采集的未处理标本，然后比较用相同技术采集的沉淀标本和离心标本。使用威尔科克森符号秩检验进行统计分析。
使用3毫米梅赛德斯套管采集的标本中，每平方毫米脂肪细胞的数量在统计学上更高（P = 0.0310）。在使用2毫米科尔曼套管（P = 0.0080）和3毫米梅赛德斯套管（P = 0.0050）的两种脂肪采集方法中，离心标本中形态改变的细胞数量在统计学上高于沉淀标本。脂肪细胞形态的改变包括细胞膜起皱、孔隙开放伴有油性物质泄漏、细胞直径减小以及细胞膜完全塌陷。
与沉淀相比，使用3毫米套管采集脂肪会产生更多的脂肪细胞，而采集的脂肪进行离心会导致形态改变的细胞数量比沉淀更多。
