Demirel Oguzhan, Karakol Percin, Bozkurt Mehmet, Tuglu Mehmet Ibrahim
Plastic, Rekonsturctive and Aesthetic Surgery Department, Istanbul Aydın University Faculty of Medicine, Istanbul, Turkey.
Department of Plastic, Reconstructive, and Aesthetic Surgery, Bagcılar Research and Training Hospital, Istanbul, Turkey.
Indian J Plast Surg. 2022 Dec 22;56(1):53-61. doi: 10.1055/s-0042-1759495. eCollection 2023 Feb.
Although fat grafts are widely used for reconstruction and aesthetic purposes, their survival rates differ significantly. Centrifugation is one of the methods used to increase the survival of fat grafts. However, experimental studies examining the long-term outcomes of centrifugation duration are currently limited. Thus, in the present study, the effects of centrifugation duration on the survival of fat grafts were assessed using an animal model. Thirty Sprague Dawley rats were included in the study and fat grafts were obtained from each specimen by excision from inguinal fat pads. Preparation protocols were administered as an en-bloc fat graft in Group 1, minced fat graft in Group 2, and fat graft centrifuged at 1,054 ×g for 2 minutes, 3 minutes, and 4 minutes in Group 3, 4, and 5, respectively. After 12 weeks of follow-up, grafts were harvested and were subjected to histopathological evaluation based on an established scoring system. En-block fat grafts were associated with necrosis, fibrosis, inflammation, vacuole formation, and alterations in adipocyte morphology. Among the three centrifugation groups, Group 3 demonstrated the best adipocyte viability and vascularity. However, graft weights decreased in all experimental groups. The centrifugation process may have positive effects on adipocyte survival by means of purifying the fat graft and increasing adipocyte concentration. When the centrifugal durations were compared, 3-minute centrifuge yielded the most favorable results.
尽管脂肪移植广泛用于重建和美容目的，但其成活率差异显著。离心是提高脂肪移植成活率的方法之一。然而，目前关于离心时间长期效果的实验研究有限。因此，在本研究中，使用动物模型评估了离心时间对脂肪移植成活率的影响。
该研究纳入了30只Sprague Dawley大鼠，通过切除腹股沟脂肪垫从每个标本获取脂肪移植体。制备方案如下：第1组为整块脂肪移植，第2组为切碎的脂肪移植，第3、4和5组分别为以1054×g离心2分钟、3分钟和4分钟的脂肪移植。随访12周后，收获移植体，并根据既定评分系统进行组织病理学评估。
整块脂肪移植与坏死、纤维化、炎症、空泡形成以及脂肪细胞形态改变有关。在三个离心组中，第3组显示出最佳的脂肪细胞活力和血管生成。然而，所有实验组的移植体重量均下降。
离心过程可能通过纯化脂肪移植体和增加脂肪细胞浓度对脂肪细胞存活产生积极影响。比较离心时间时，3分钟离心产生的结果最有利。
