School of Engineering and Applied Sciences and Department of Physics, Harvard University, Cambridge, MA, 02138, USA.
Small. 2014 Mar 12;10(5):950-6. doi: 10.1002/smll.201301904. Epub 2013 Oct 22.
A microfluidic approach is reported for the high-throughput, continuous production of giant unilamellar vesicles (GUVs) using water-in-oil-in-water double emulsion drops as templates. Importantly, these emulsion drops have ultrathin shells; this minimizes the amount of residual solvent that remains trapped within the GUV membrane, overcoming a major limitation of typical microfluidic approaches for GUV fabrication. This approach enables the formation of microdomains, characterized by different lipid compositions and structures within the GUV membranes. This work therefore demonstrates a straightforward and versatile approach to GUV fabrication with precise control over the GUV size, lipid composition and the formation of microdomains within the GUV membrane.
本文报道了一种使用油包水双乳液滴作为模板，高通量、连续制备大单室囊泡（GUV）的微流控方法。重要的是，这些乳液滴具有超薄的外壳；这最大限度地减少了残留在 GUV 膜内的溶剂量，克服了典型微流控方法制备 GUV 的一个主要限制。该方法能够在 GUV 膜内形成具有不同脂质组成和结构的微区。因此，这项工作展示了一种简单而通用的 GUV 制备方法，可以精确控制 GUV 的大小、脂质组成以及 GUV 膜内微区的形成。
