Seo Hanjin, Lee Hyomin
Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, South Korea.
Biomicrofluidics. 2021 Mar 30;15(2):021301. doi: 10.1063/5.0048441. eCollection 2021 Mar.
Recent advances in droplet microfluidics have led to the fabrication of versatile vesicles with a structure that mimics the cellular membrane. These artificial cell-like vesicles including polymersomes and liposomes effectively enclose an aqueous core with well-defined size and composition from the surrounding environment to implement various biological reactions, serving as a diverse functional reactor. The advantage of realizing various biological phenomena within a compartment separated by a membrane that resembles a natural cell membrane is actively explored in the fields of synthetic biology as well as biomedical applications including drug delivery, biosensors, and bioreactors, to name a few. In this Perspective, we first summarize various methods utilized in producing these polymersomes and liposomes. Moreover, we will highlight some of the recent advances in the design of these artificial cell-like vesicles for functional bioreactors and discuss the current issues and future perspectives.
液滴微流控技术的最新进展已促成了具有模仿细胞膜结构的多功能囊泡的制造。这些人工细胞样囊泡，包括聚合物囊泡和脂质体，能有效地将具有明确大小和组成的水相核心与周围环境隔离开来，以实现各种生物反应，充当多样化的功能反应器。在合成生物学以及包括药物递送、生物传感器和生物反应器等在内的生物医学应用领域，人们正在积极探索在由类似天然细胞膜的膜分隔的隔室内实现各种生物现象的优势。在这篇综述中，我们首先总结了用于制备这些聚合物囊泡和脂质体的各种方法。此外，我们将重点介绍这些人工细胞样囊泡用于功能生物反应器设计的一些最新进展，并讨论当前的问题和未来的前景。
