Pelzer D, Cavalié A, Trautwein W
Basic Res Cardiol. 1985;80 Suppl 2:65-9.
Ca channels in excitable membranes are of great importance for many cellular functions. Modulation of these channels by voltage, neurotransmitters and drugs regulates Ca influx into the cell and thereby alters the functional state of the cell (7). For many years, quantitative analysis has been seriously complicated by experimental problems (see 2, 8, 10). There has been no preparation as suitable and no drug as specific for Ca channels, as the squid axon and TTX are for Na channels (10). The advent of the isolated adult heart cell and the development of powerful new electrophysiological methods, however, have opened up the possibility to record Ca channel activity at the level of single cells and single channels and to obtain evidence on molecular mechanisms of Ca channel gating and modulation.
可兴奋膜中的钙通道对许多细胞功能极为重要。电压、神经递质和药物对这些通道的调节可控制钙离子流入细胞，进而改变细胞的功能状态（7）。多年来，实验问题严重困扰着定量分析（见2、8、10）。一直没有像乌贼轴突和河豚毒素对于钠通道那样适合的标本以及针对钙通道的特异性药物（10）。然而，分离的成年心脏细胞的出现以及强大的新电生理方法的发展，为在单细胞和单通道水平记录钙通道活性以及获取钙通道门控和调节分子机制的证据开辟了可能性。
