一种测定“需氧转氢酶”活性的动力学方法（作者译）
[A kinetic method for the determination of the activity of "aerobic transhydrogenases" (author's transl)].
作者信息
Majkić N, Djordjević-Spasić S, Berkes I
出版信息
Clin Chim Acta. 1975 Dec 1;65(2):227-33. doi: 10.1016/0009-8981(75)90112-6.
The chromogen ABTS is the di-ammonium salt of 2,2'-azino-di[3-ethyl-benzthiazolin-sulfonic acid (6)] routinely used in the "glucose-oxidase assay" with the peroxidase (GOD-Perid method, Boehringer). 1. The specific property of ABTS to give a stable radical cation by oxidation with hydrogen peroxide in the presence of peroxidase was used to design a kinetic method, for enzyme-activity determinations. 2. The assay is suitable for the specific oxido-reductase using oxygen as acceptor, known also as "aerobic transhydrogenases" which are H2O2 formers (EC 1.-.3.-). 3. L-Amino acid: oxygen oxidoreductase (deaminating) (EC 1.4.3.2), was used throughout, being a representative model for such determinations.
显色剂ABTS是2,2'-叠氮二[3-乙基苯并噻唑啉磺酸(6)]的二铵盐，常用于“葡萄糖氧化酶测定法”中的过氧化物酶法（GOD-Perid法，勃林格公司）。1. ABTS在过氧化物酶存在下被过氧化氢氧化生成稳定的自由基阳离子这一特性被用于设计一种动力学方法来测定酶活性。2. 该测定法适用于以氧作为受体的特定氧化还原酶，这类酶也被称为“需氧转氢酶”，它们是过氧化氢的生成者（酶学委员会编号1.-.3.-）。3. 整个实验使用了L-氨基酸：氧氧化还原酶（脱氨基）（酶学委员会编号1.4.3.2），它是此类测定的一个代表性模型。
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