以2,2'-叠氮基二-(3-乙基苯并噻唑啉-6-磺酸)为显色剂时过氧化物酶的稳态动力学
The steady-state kinetics of peroxidase with 2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen.
作者信息
Childs R E, Bardsley W G
出版信息
Biochem J. 1975 Jan;145(1):93-103. doi: 10.1042/bj1450093.
Abstract
The chemical nature of the important new chromogen ABTS [2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid)] is described together with an account of the redox and spectroscopic properties of the system ABTS--H2O2--peroxidase. Keq. is calculated and a study of the steady-state kinetics over a whole range of substrate concentrations is reported. By using novel methods of kinetic analysis, an interpretation of the results is given which requires some extension of the classical peroxidase mechanism.
摘要
描述了重要的新型显色剂ABTS[2,2'-偶氮二-(3-乙基苯并噻唑啉-6-磺酸)]的化学性质，并介绍了ABTS-H₂O₂-过氧化物酶体系的氧化还原和光谱性质。计算了平衡常数，并报道了在整个底物浓度范围内对稳态动力学的研究。通过使用新型动力学分析方法，对结果进行了解释，这需要对经典过氧化物酶机制进行一些扩展。
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