Schwerdtfeger W K, Danscher G, Geiger H
Brain Res. 1985 Nov 25;348(1):69-76. doi: 10.1016/0006-8993(85)90361-0.
The Timm and selenium staining techniques, based on silver amplification of endogenous zinc, produce an electron-dense precipitate in boutons. The staining characteristics of the two methods were compared by examining two allocortical regions, prepiriform cortex and entorhinal cortex, in the brain of the European hedgehog. In the 3 layers of prepiriform cortex and the 6 layers of entorhinal cortex the methods revealed sublayers, which allows a precise delimitation of areas 28M, 28L, a short transition zone, and the prepiriform cortex. The lamination of the entorhinal cortex of the hedgehog is similar to that found in the rat, but appears less distinct. This may point to a lower degree of afferent organization. For light microscopical investigations, the selenium technique appears superior to Timm's method because it produces a more distinct zonation pattern.
基于内源性锌的银放大作用的蒂姆（Timm）染色技术和硒染色技术，会在终扣中产生电子致密沉淀物。通过检查欧洲刺猬大脑中的两个异皮质区域，即梨前皮质和内嗅皮质，对这两种方法的染色特征进行了比较。在梨前皮质的3层和内嗅皮质的6层中，这些方法揭示了亚层，这使得能够精确界定28M区、28L区、一个短过渡区和梨前皮质。刺猬内嗅皮质的分层与大鼠的相似，但不太明显。这可能表明传入组织的程度较低。对于光学显微镜研究，硒技术似乎优于蒂姆方法，因为它产生的分区模式更明显。
