Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China.
Bioresour Technol. 2013 Dec;150:73-8. doi: 10.1016/j.biortech.2013.09.130. Epub 2013 Oct 7.
A combination process of alkali impregnation and refining was used as a pretreatment to improve the production of fermentable sugar. The surface structures and crystallinities of wood samples were characterized to explain the relationships between the pretreatment action and enzymatic efficiency. After refining, the reducing sugar contents in hydrolyzates were analyzed by UV-Vis and HPLC. The results showed that the enzymatic efficiency could be improved by the combined pretreatment, due to the increment of specific surface area and the release of more free hydroxyls. Comparing to the sodium hydroxide and deionized water, the impregnation with magnesium hydroxide had low refining energy consumption and high yield of reducing sugar (glucose and xylose) in enzymolysis process, where about 560 kWh/t of the energy was saved in refining, and the yield of the reducing sugar was as high as 91.53%. And the enzymolysis could be improved by a certain amount of magnesium ions.
碱浸渍与精炼相结合的预处理方法被用于提高发酵糖的产量。对木材样品的表面结构和结晶度进行了表征，以解释预处理作用与酶效率之间的关系。精炼后，通过 UV-Vis 和 HPLC 分析水解产物中的还原糖含量。结果表明，由于比表面积的增加和更多游离羟基的释放，复合预处理可以提高酶效率。与氢氧化钠和去离子水相比，用氢氧化镁浸渍具有较低的精炼能耗和较高的酶解还原糖（葡萄糖和木糖）得率，其中精炼过程可节约约 560 kWh/t 的能量，还原糖得率高达 91.53%。并且一定量的镁离子可以改善酶解过程。
