Department of Mechatronic Engineering, Huafan University, Shihding, Taipei Hsien 223-01, Taiwan.
Sensors (Basel). 2013 Oct 21;13(10):14161-74. doi: 10.3390/s131014161.
The purpose of this study was to develop novel nanoscale biosensors using titania nanotubes (TNTs) made by anodization. Titania nanotubes were produced on pure titanium sheets by anodization at room temperature. In this research, the electrolyte composition ethylene glycol 250 mL/NH4F 1.5 g/DI water 20 mL was found to produce the best titania nanotubes array films for application in amperometric biosensors. The amperometric results exhibit an excellent linearity for uric acid (UA) concentrations in the range between 2 and 14 mg/dL, with 23.3 (µA·cm-2)·(mg/dL)-1 UA sensitivity, and a correlation coefficient of 0.993. The glucose biosensor presented a good linear relationship in the lower glucose concentration range between 50 and 125 mg/dL, and the corresponding sensitivity was approximately 249.6 (µA·cm-2)·(100 mg/dL)-1 glucose, with a correlation coefficient of 0.973.
本研究旨在开发新型纳米级生物传感器，使用阳极氧化法制备的二氧化钛纳米管（TNTs）。通过室温下的阳极氧化，在纯钛片上制备出二氧化钛纳米管。在本研究中，发现电解液组成为乙二醇 250 mL/NH4F 1.5 g/DI 水 20 mL，可产生最佳的二氧化钛纳米管阵列薄膜，适用于安培型生物传感器。安培测量结果显示尿酸（UA）浓度在 2 至 14 mg/dL 范围内具有极好的线性关系，UA 灵敏度为 23.3 (µA·cm-2)·(mg/dL)-1，相关系数为 0.993。葡萄糖生物传感器在较低的葡萄糖浓度范围（50 至 125 mg/dL）内呈现出良好的线性关系，相应的灵敏度约为 249.6 (µA·cm-2)·(100 mg/dL)-1 葡萄糖，相关系数为 0.973。
