通过空间和时间分辨拉曼微光谱法监测体外骨结节的矿化。
Monitoring the mineralisation of bone nodules in vitro by space- and time-resolved Raman micro-spectroscopy.
机构信息
School of Physics and Astronomy, University of Nottingham, Nottingham, NG7 2RD, UK.
出版信息
Analyst. 2014 Jan 7;139(1):55-8. doi: 10.1039/c3an01716h. Epub 2013 Oct 24.
PMID:24153382
Abstract
Raman microscopy was used as a label-free method to study the mineralisation of bone nodules formed by mesenchymal stem cells cultured in osteogenic medium in vitro. Monitoring individual bone nodules over 28 days revealed temporal and spatial changes in the crystalline phase of the hydroxyapatite components of the nodules.
摘要
拉曼显微镜被用作一种无标记的方法，来研究体外培养在成骨培养基中的间充质干细胞形成的骨结节的矿化。对单个骨结节进行 28 天的监测，揭示了结节中羟基磷灰石成分的晶体相的时间和空间变化。
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