Center for Solid State Physics and New Materials, Institute of Physics Belgrade, University of Belgrade, Pregrevica 118, Belgrade 11080, Serbia.
Laboratory for Experimental Hematology and Stem Cells, Institute for Medical Research, University of Belgrade, Belgrade 11000, Serbia.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Apr 15;213:384-390. doi: 10.1016/j.saa.2019.01.069. Epub 2019 Jan 29.
We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.
我们采用微拉曼光谱技术深入了解从牙周韧带中分离出的单个间充质干细胞的固有生物分子特征。此外，我们还刺激这些细胞向脂肪细胞、软骨细胞和成骨细胞谱系分化，并使用微拉曼光谱技术评估其分化状态。在这两种情况下，由于玻璃盖玻片的广泛可用性和成本效益，我们都将其用作基质。在所有样本组中，都观察到相同类型的行为，表现为拉曼光谱的变化：蛋白质/脂质带的相对强度增加，核酸带的相对强度降低。我们进行了主成分分析形式的综合统计分析，结果表明具有相似特征的细胞明显分组。尽管原代干细胞及其分化谱系存在不均匀性，但我们证明微拉曼光谱足以区分细胞的状态，这对于医学和临床应用可能具有重要价值。
