Pfeifle J, Boller K, Anderer F A
Exp Cell Res. 1986 Jan;162(1):11-22. doi: 10.1016/0014-4827(86)90422-2.
The association of phosphoproteins pp135 and pp105 with distinct substructures of the nucleolus was studied by cytochemical and immunological methods at the light microscopic and electron microscopic level. Both phosphoproteins exhibited a very high affinity for silver and Giemsa staining compared to other nucleolar proteins. Immunolocalization of pp135 and pp105 during mitosis by light microscopy revealed a tight association of pp135 with the silver staining nucleolus organizer region (NOR), whereas pp105 (cross-reacting with C23) appeared to be only partially associated with the NOR, exclusively at telophase. At the immunoelectron microscopic level the distribution of pp135 and pp105 was investigated in interphase nucleoli. Phosphoprotein pp135 was located in the fibrillar shell and pp105 in the fibrillar shell and the granular zone. The fibrillar centers were essentially free of both phosphoproteins..
通过细胞化学和免疫学方法，在光学显微镜和电子显微镜水平上研究了磷蛋白pp135和pp105与核仁不同亚结构的关联。与其他核仁蛋白相比，这两种磷蛋白对银染和吉姆萨染色都表现出非常高的亲和力。光学显微镜下对有丝分裂期间pp135和pp105的免疫定位显示，pp135与银染核仁组织区（NOR）紧密相关，而pp105（与C23交叉反应）似乎仅在末期与NOR部分相关。在免疫电子显微镜水平上，研究了间期核仁中pp135和pp105的分布。磷蛋白pp135位于纤维状外壳中，pp105位于纤维状外壳和颗粒区。纤维中心基本上没有这两种磷蛋白。
