Spector D L, Ochs R L, Busch H
Chromosoma. 1984;90(2):139-48. doi: 10.1007/BF00292451.
Nucleolar organizer region (NOR)-specific silver staining and immunolocalization of nucleolar phosphoproteins B23 and C23 were compared in Novikoff hepatoma ascites cells. Silver staining and protein C23 immunostaining were both localized in the fibrillar shell surrounding the fibrillar center and in the fibrillar center. During mitosis, silver staining and protein C23 were localized at the NORs. Therefore, protein C23 and the silver-staining protein both seem to be associated with rDNA-containing structures (Mirre and Stahl 1981). A comparison of toluidine blue staining specific for RNA and B23 immunostaining demonstrated that protein B23 was associated with RNA-containing regions of the nucleolus and was absent from the fibrillar centers. Localization of these proteins and their functions are discussed in relation to the organization of the nucleolus.
在诺维科夫肝癌腹水细胞中，比较了核仁组织区（NOR）特异性银染以及核仁磷蛋白B23和C23的免疫定位。银染和蛋白C23免疫染色均定位于围绕纤维中心的纤维壳以及纤维中心内。在有丝分裂期间，银染和蛋白C23定位于核仁组织区。因此，蛋白C23和银染蛋白似乎都与含rDNA的结构相关（米尔和施塔尔，1981年）。对RNA特异性的甲苯胺蓝染色和B23免疫染色的比较表明，蛋白B23与核仁中含RNA的区域相关，而在纤维中心不存在。结合核仁的组织结构对这些蛋白质的定位及其功能进行了讨论。
