Thomson T B, Crider B P, Eagon R G
J Antimicrob Chemother. 1985 Aug;16(2):157-63. doi: 10.1093/jac/16.2.157.
Dihydrostreptomycin was taken up in isolated cytoplasmic membrane vesicles of Pseudomonas putida by an active transport mechanism. Saturation kinetics were observed with an apparent Km and Vmax of 15 mM and 50 nmol/min/mg of protein respectively. The evidence suggested that the observed kinetics was that of the energy-dependent phase I component of dihydrostreptomycin uptake. Neither magnesium nor the polyamine, spermine, inhibited dihydrostreptomycin transport. Thus, the inhibition of aminoglycoside uptake in intact cells of Gram-negative bacteria and the increase in the minimal inhibitory concentration in the presence of multivalent cations and polyamines were interpreted to be effects that take place at the outer membrane level.
二氢链霉素通过主动转运机制被恶臭假单胞菌的分离细胞质膜囊泡摄取。观察到饱和动力学，表观 Km 和 Vmax 分别为 15 mM 和 50 nmol/分钟/毫克蛋白质。证据表明，观察到的动力学是二氢链霉素摄取的能量依赖 I 相成分的动力学。镁和多胺精胺均未抑制二氢链霉素的转运。因此，革兰氏阴性菌完整细胞中氨基糖苷摄取的抑制以及在多价阳离子和多胺存在下最小抑菌浓度的增加被解释为发生在外膜水平的效应。
