Patil Shankargouda, Premalatha Br, Rao Roopa S, Ganavi Bs
Department of Oral Pathology, M S Ramaiah Dental College, Bangalore, India.
J Int Oral Health. 2013 Feb;5(1):31-8. Epub 2013 Feb 26.
The quest for formalin substitutes has long been going on due to its health hazards. Honey has been proven as a safe alternative to formalin. However, we explored more economical, eco-friendly & readily available substances like sugar & jaggery as natural substitutes for formalin. The aim of this study was to compare the tissue fixation abilities of honey, sugar syrup & jaggery syrup with that of formalin using H & E stain and to determine the best fixative among the three.
Commercially available fresh goat meat (buccal mucosa) was cut into five bits & each bit was placed in five different containers containing 10% buffered formalin, distilled water, 20% honey, 20% sugar syrup & 30% jaggery syrup with formalin as positive control & distilled water as negative control. 24 hours tissue fixation was attained at room temperature followed by conventional processing and staining. The tissue sections were assessed for cytoplasmic, nuclear details & staining quality under light microscopy. Each criteria was rated on a scale of 1- 4 (1 for poor & 4 for excellent) & the whole procedure was blinded. RESULTS were analysed by Kruskal Wallis ANOVA test. Inter-observer variability was determined by Kappa statistics.
The preservation of tissue by honey, sugar & jaggery syrup was comparable to that of formalin. Among the three natural fixatives, jaggery syrup excelled.
Our effort of using sugar and jaggery for tissue fixation is first of its kind and yielded good results. Hence they can be successfully adopted in routine histopathology laboratories in place of formalin.
Natural fixatives can be used in place of the hazardous formalin with equal efficiency. Also, jaggery being highly economical and universally available can be employed in large scale as in screening camps. How to cite this article: Patil S, Premalatha B R, Rao R S, Ganavi B S. Revelation in the Field of Tissue Preservation - A Preliminary Study on Natural Formalin Substitutes. J Int Oral Health 2013; 5(1):31-38.
由于福尔马林对健康有害，长期以来人们一直在寻找其替代品。蜂蜜已被证明是福尔马林的一种安全替代品。然而，我们探索了更经济、环保且易于获得的物质，如糖和粗糖，作为福尔马林的天然替代品。本研究的目的是使用苏木精和伊红染色（H&E染色）比较蜂蜜、糖浆和粗糖浆与福尔马林的组织固定能力，并确定三者中最佳的固定剂。
将市售新鲜羊肉（颊黏膜）切成五小块，每块分别置于五个不同容器中，容器中分别装有10%缓冲福尔马林、蒸馏水、20%蜂蜜、20%糖浆和30%粗糖浆，以福尔马林作为阳性对照，蒸馏水作为阴性对照。在室温下进行24小时组织固定，随后进行常规处理和染色。在光学显微镜下评估组织切片的细胞质、细胞核细节及染色质量。每个标准按1 - 4分进行评分（1分为差，4分为优），整个过程采用盲法。结果通过Kruskal Wallis方差分析检验进行分析。观察者间的变异性通过Kappa统计量确定。
蜂蜜、糖和粗糖浆对组织的保存效果与福尔马林相当。在三种天然固定剂中，粗糖浆表现最佳。
我们使用糖和粗糖进行组织固定的尝试尚属首次，且取得了良好效果。因此，它们可以成功地在常规组织病理学实验室中替代福尔马林使用。
天然固定剂可以以相同效率替代有害的福尔马林。此外，粗糖价格低廉且普遍可得，可在大规模筛查活动中广泛应用。如何引用本文：Patil S, Premalatha B R, Rao R S, Ganavi B S. 组织保存领域的新发现——天然福尔马林替代品的初步研究。《国际口腔健康杂志》2013年；5(1):31 - 38。
