Pandiar Deepak, Baranwal Harakh Chand, Kumar Sandip, Ganesan Vellaichamy, Sonkar Piyush Kumar, Chattopadhyay Kausik
Department of Dentistry, Institute of Medical Sciences, Varanasi, Uttar Pradesh, India.
Pathology, Institute of Medical Sciences, Varanasi, Uttar Pradesh, India.
J Oral Maxillofac Pathol. 2017 May-Aug;21(2):317. doi: 10.4103/jomfp.JOMFP_224_15.
Ethanol has satisfactorily been used as cyto-fixative. Owing to its limitations, pathologists have always searched for new fixatives. The present study was conducted to evaluate the efficacy of two natural sweeteners as cyto-fixatives. An attempt has also been made to understand the underlying mechanism by which these fixatives fix the oral mucosal cells.
Three smears were collected from 25 healthy volunteers. One smear was fixed in ethanol and the other two in 20% aqueous honey solution and 30% aqueous jaggery solution for 15-30 min followed by Papanicolaou staining. Slides were evaluated for nuclear staining, cytoplasmic staining, cell morphology, clarity of staining and uniformity of staining randomly irrespective of the fixatives. Chi-square test and Bonferroni test were done using SPSS software. < 0.05 was considered to be statistically significant. In addition, cytological fixatives were analyzed for the pH and amount of reducing sugars.
It was found that for all the characteristics studied, no statistically significant difference was seen between the three fixatives. The pH of the both tested fixatives remained acidic even after 1 week. The amount of reducing sugars in 20% aqueous honey solution and 30% aqueous jaggery solution was 19.3 g/100 mL and 2.07 g/100 mL, respectively.
Both the test fixatives gave results equivalent to ethanol and thus can be used as alternative fixatives for oral smears. It is proposed that 20% aqueous honey and 30% aqueous jaggery fix the oral smears satisfactorily in a mechanism akin to ethanol by coagulating and denaturing proteins.
乙醇一直被令人满意地用作细胞固定剂。由于其局限性，病理学家一直在寻找新的固定剂。本研究旨在评估两种天然甜味剂作为细胞固定剂的效果。同时还尝试了解这些固定剂固定口腔黏膜细胞的潜在机制。
从25名健康志愿者身上采集了三张涂片。一张涂片用乙醇固定，另外两张分别用20%的蜂蜜水溶液和30%的粗糖水溶液固定15 - 30分钟，随后进行巴氏染色。不论固定剂如何，随机对玻片进行核染色、细胞质染色、细胞形态、染色清晰度和染色均匀性的评估。使用SPSS软件进行卡方检验和邦费罗尼检验。P < 0.05被认为具有统计学意义。此外，还对细胞固定剂的pH值和还原糖含量进行了分析。
发现对于所有研究的特征，三种固定剂之间没有统计学上的显著差异。两种测试固定剂的pH值即使在1周后仍保持酸性。20%的蜂蜜水溶液和30%的粗糖水溶液中的还原糖含量分别为19.3 g/100 mL和2.07 g/100 mL。
两种测试固定剂的效果与乙醇相当，因此可作为口腔涂片的替代固定剂。有人提出，20%的蜂蜜水溶液和30%的粗糖水溶液通过使蛋白质凝固和变性，以类似于乙醇的机制令人满意地固定口腔涂片。
