Laboratory of Insect Toxicology, Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, People's Republic of China.
PLoS One. 2013 Oct 14;8(10):e77295. doi: 10.1371/journal.pone.0077295. eCollection 2013.
Rhodojaponin-III is a nonvolatile botanical grayanoid diterpene compound, which has antifeedant and oviposition deterrence effects against many kinds of insects. However, the molecular mechanism of the chemoreception process remains unknown. In this study, the important role of BdorCSP2 in the recognition of Rhodojaponin-III was identified. The full length cDNA encoding BdorCSP2 was cloned from legs of Bactrocera dorsalis. The results of expression pattern revealed that BdorCSP2 was abundantly expressed in the legs of adult B. dorsalis. Moreover, the expression of BdorCSP2 could be up-regulated by Rhodojaponin-III. In order to gain comprehensive understanding of the recognition process, the binding affinity between BdorCSP2 and Rhodojaponin-III was measured by fluorescence binding assay. Silencing the expression of BdorCSP2 through the ingestion of dsRNA could weaken the effect of oviposition deterrence and antifeedant of Rhodojaponin-III. These results suggested that BdorCSP2 of B. dorsalis could be involved in chemoreception of Rhodojaponin-III and played a critical role in antifeedant and oviposition behaviors induced by Rhodojaponin-III.
罗通定-III 是一种非挥发性植物格雷森二萜化合物，对许多种昆虫具有拒食和产卵驱避作用。然而，其化学感受过程的分子机制尚不清楚。在本研究中，鉴定了 BdorCSP2 在罗通定-III 识别中的重要作用。从桔小实蝇（Bactrocera dorsalis）的腿中克隆了编码 BdorCSP2 的全长 cDNA。表达模式的结果表明，BdorCSP2 在桔小实蝇成虫的腿中大量表达。此外，罗通定-III 可以上调 BdorCSP2 的表达。为了全面了解识别过程，通过荧光结合测定测量了 BdorCSP2 与罗通定-III 的结合亲和力。通过摄入 dsRNA 沉默 BdorCSP2 的表达会削弱罗通定-III 的产卵驱避和拒食作用。这些结果表明，桔小实蝇的 BdorCSP2 可能参与罗通定-III 的化学感受，并在罗通定-III 诱导的拒食和产卵行为中发挥关键作用。
