小鼠IgM中的截短μ（μ'）链。μ'链缺乏可变区的证据。
Truncated mu (mu') chains in murine IgM. Evidence that mu' chains lack variable regions.
作者信息
Marks R, Bosma M J
出版信息
J Exp Med. 1985 Dec 1;162(6):1862-77. doi: 10.1084/jem.162.6.1862.
Abstract
Secreted IgM was shown to contain truncated mu (mu') chains with an apparent molecular mass of approximately 55 kD. The estimated percentage of IgM heavy (H) chains in the mu' form ranged from less than or equal to 1% in the case of one tumor IgM protein (104E) to greater than or equal to 30% in normal serum IgM. Serum mu' chains lacked antigenic determinants characteristic of immunoglobulin variable regions and showed a restricted isoelectric focusing pattern compared with that of conventional mu chains. Intracellular mu' chains were readily detected in bone marrow cells but not in spleen or lymph node cells; mu' chains were also detected in IgM-producing tumor cells and in a hybridoma cell line that deleted its productive mu allele. These results predict irregularities in IgM structure and recall an old controversy concerning the valence of IgM molecules.
摘要
分泌型IgM被证明含有截短的μ（μ'）链，其表观分子量约为55kD。μ'形式的IgM重链（H链）估计百分比范围从一种肿瘤IgM蛋白（104E）的小于或等于1%到正常血清IgM的大于或等于30%。血清μ'链缺乏免疫球蛋白可变区特有的抗原决定簇，与传统μ链相比，其等电聚焦模式受限。在骨髓细胞中很容易检测到细胞内μ'链，但在脾或淋巴结细胞中未检测到；在产生IgM的肿瘤细胞和缺失其有功能μ等位基因的杂交瘤细胞系中也检测到了μ'链。这些结果预示着IgM结构的不规则性，并让人想起一个关于IgM分子价态的古老争议。
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