United States Department of Agriculture, Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Plant Sciences Institute, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, MD 20705, USA.
Insect Biochem Mol Biol. 2013 Dec;43(12):1161-71. doi: 10.1016/j.ibmb.2013.10.005. Epub 2013 Oct 22.
The yellow-fever mosquito, Aedes aegypti, infects a growing number of people every year with dengue, yellow fever and chikungunya viruses. Contact chemoreception in mosquitoes influences a number of behaviors including host-selection, oviposition and feeding. While these behaviors are in many instances well documented, the molecular mechanisms mediating them are not well understood. Here we report the results of sequencing total messenger RNA in the labella and tarsi of both male and female Ae. aegypti to reveal Gustatory Receptor (GR) gene expression profiles in these major gustatory appendages. Gene expression levels in each tissue were verified by RT-qPCR. We discuss potential functions for the GRs revealed here by considering homologous GRs in other insects. Specific GRs provide molecular targets for modification of gustatory-mediated behaviors in this important disease vector.
黄热病蚊子，埃及伊蚊，每年都有越来越多的人感染登革热、黄热病和基孔肯雅热病毒。蚊子的接触化学感受影响许多行为，包括宿主选择、产卵和进食。虽然这些行为在许多情况下都有详细记录，但介导这些行为的分子机制尚不清楚。在这里，我们报告了对实验室和实验室的总信使 RNA 进行测序的结果雄性和雌性埃及伊蚊的跗节，以揭示这些主要味觉附肢中的味觉受体 (GR) 基因表达谱。通过 RT-qPCR 验证了每种组织中的基因表达水平。我们通过考虑其他昆虫中的同源 GR 来讨论这里揭示的 GR 的潜在功能。特定的 GR 为修饰这种重要病媒传播的味觉介导行为提供了分子靶标。
