SINTEF Materials and Chemistry, Department of Biotechnology, Sem Sælands vei 2a, 7465, Trondheim, Norway.
Appl Microbiol Biotechnol. 2014 Jan;98(2):603-10. doi: 10.1007/s00253-013-5320-0. Epub 2013 Oct 26.
Phenazine natural products/compounds possess a range of biological activities, including anti-microbial and cytotoxic, making them valuable starting materials for drug development in several therapeutic areas. These compounds are biosynthesized almost exclusively by eubacteria of both terrestrial and marine origins from erythrose 4-phosphate and phosphoenol pyruvate via the shikimate pathway. In this paper, we report isolation of actinomycete bacteria from marine sediment collected in the Trondheimfjord, Norway. Screening of the isolates for biological activity produced several "hits", one of which was followed up by identification and purification of the active compound from the actinomycete bacterium Streptosporangium sp. The purified compound, identified as 1,6-dihydroxyphenazine-5,10-dioxide (iodinin), was subjected to extended tests for biological activity against bacteria, fungi and mammalian cells. In these tests, the iodinin demonstrated high anti-microbial and cytotoxic activity, and was particularly potent against leukaemia cell lines. This is the first report on the isolation of iodinin from a marine-derived Streptosporangium.
吩嗪类天然产物/化合物具有多种生物活性，包括抗微生物和细胞毒性，使其成为几个治疗领域药物开发的有价值的起始原料。这些化合物几乎完全由陆地和海洋来源的真细菌通过莽草酸途径从赤藓糖 4-磷酸和磷酸烯醇丙酮酸生物合成。在本文中，我们报告了从挪威特隆赫姆峡湾采集的海洋沉积物中分离出放线菌细菌。对分离物进行生物活性筛选产生了几种“命中”，其中一种通过鉴定和纯化放线菌细菌 Streptosporangium sp. 中的活性化合物进行了跟进。从放线菌细菌 Streptosporangium sp. 中分离出的纯化化合物被鉴定为 1,6-二羟基吩嗪-5,10-二氧化物（碘菌素），并对其进行了针对细菌、真菌和哺乳动物细胞的生物活性的扩展测试。在这些测试中，碘菌素表现出高抗微生物和细胞毒性活性，并且对白血病细胞系特别有效。这是首次从海洋衍生的 Streptosporangium 中分离出碘菌素的报道。
