Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan.
Techno-Suruga Laboratory Co., Ltd, Shizuoka 424-0065, Japan.
Int J Syst Evol Microbiol. 2014 Feb;64(Pt 2):449-455. doi: 10.1099/ijs.0.055186-0. Epub 2013 Oct 24.
Gram-staining-positive anaerobic rods were isolated from the faeces of a wild lowland gorilla (Gorilla gorilla gorilla) in Moukalaba-Doudou National Park, Gabon, and strain GG01(T) was taxonomically investigated. Based on phylogenetic analyses and specific phenotypic characteristics, the strain belonged to the genus Bifidobacterium. Phylogenetic analysis of its 16S rRNA gene sequence revealed that strain GG01(T) formed a single monophyletic cluster and had a distinct line of descent. Based on 16S rRNA gene sequence similarity, the type strains of Bifidobacterium catenulatum JCM 1194(T) (98.3%) and Bifidobacterium pseudocatenulatum (98.1%) JCM 1200(T) were the most closely related to this novel strain, although it was clear that they belonged to different species. hsp60 sequences also supported these relationships. The DNA G+C content of this novel strain was 60.1 mol%. Bifidobacterium moukalabense sp. nov. (type strain GG01(T) = JCM 18751(T) = DSM 27321(T)) is proposed.
从加蓬穆卡巴拉-杜杜国家公园的一只野生低地大猩猩（Gorilla gorilla gorilla）粪便中分离到革兰氏染色阳性的厌氧杆菌，并对菌株 GG01(T)进行了分类学研究。基于系统发育分析和特定的表型特征，该菌株属于双歧杆菌属。16S rRNA 基因序列的系统发育分析表明，菌株 GG01(T)形成了一个单一的单系聚类，具有明显的进化枝。根据 16S rRNA 基因序列的相似性，双歧杆菌 catenulatum JCM 1194(T)（98.3%）和双歧杆菌 pseudocatenulatum（98.1%）JCM 1200(T)的模式菌株与该新菌株最为密切相关，尽管它们显然属于不同的种。hsp60 序列也支持这些关系。该新菌株的 DNA G+C 含量为 60.1 mol%。建议将其命名为穆卡巴拉双歧杆菌新种（模式菌株 GG01(T)=JCM 18751(T)=DSM 27321(T)）。
