Department of Systems Biotechnology, Chung-Ang University, Anseong 456-756, Republic of Korea.
Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3134-3139. doi: 10.1099/ijs.0.063479-0. Epub 2014 Jun 18.
A novel strain, designated strain CU3-7(T), was isolated from faeces of a two-week-old baby. The isolate was Gram-staining-positive, anaerobic and rod-shaped. Results from 16S rRNA gene sequence analysis revealed that strain CU3-7(T) was phylogenetically affiliated with members of the genus Bifidobacterium. Strain CU3-7(T) showed the highest level of sequence similarity with Bifidobacterium adolescentis KCTC 3216(T) (98.4 %), followed by Bifidobacterium ruminantium KCTC 3425(T) (97.9 %). Analysis of hsp60 sequences showed that strain CU3-7(T) was closely related to B. adolescentis KCTC 3216(T) (94.0 %) and B. ruminantium KCTC 3425(T) (92.5 %). The DNA-DNA hybridization values with the closely related strains were all below the cut-off value for species delineation, 17.0 % with B. ruminantium KCTC 3425(T) and 14.9 % with B. adolescentis KCTC 3216(T). Fructose-6-phosphate phosphoketolase activity was detected. The predominant cellular fatty acids were C16 : 0 (27.7 %), C18 : 1ω9c (27.4 %) and C18 : 1ω9c dimethylacetate (15.5 %). The DNA G+C content was 58.6 mol%. On the basis of polyphasic taxonomy, strain CU3-7(T) should be classified as the type strain of a novel species within the genus Bifidobacterium, for which the name Bifidobacterium faecale sp. nov. is proposed ( = KACC 17904(T) = JCM 19861(T)).
一株新型菌株，命名为 CU3-7(T) 株，从一名两周大婴儿的粪便中分离得到。该分离株革兰氏染色阳性，为厌氧菌，呈杆状。16S rRNA 基因序列分析结果表明，CU3-7(T) 株与双歧杆菌属的成员具有系统发育相关性。CU3-7(T) 株与双歧杆菌青春亚种 KCTC 3216(T)（98.4%）的序列相似性最高，其次是双歧杆菌反刍亚种 KCTC 3425(T)（97.9%）。hsp60 序列分析表明，CU3-7(T) 株与双歧杆菌青春亚种 KCTC 3216(T)（94.0%）和双歧杆菌反刍亚种 KCTC 3425(T)（92.5%）密切相关。与密切相关菌株的 DNA-DNA 杂交值均低于种间区分的界限值（17.0%与双歧杆菌反刍亚种 KCTC 3425(T)，14.9%与双歧杆菌青春亚种 KCTC 3216(T)）。检测到果糖-6-磷酸磷酸酮醇酶活性。主要细胞脂肪酸为 C16:0（27.7%）、C18:1ω9c（27.4%）和 C18:1ω9c 二甲酯（15.5%）。DNA G+C 含量为 58.6 mol%。基于多相分类学，CU3-7(T) 株应归类为双歧杆菌属的一个新型种的模式株，建议将其命名为粪双歧杆菌（Bifidobacterium faecale sp. nov.）。（=KACC 17904(T)=JCM 19861(T)）。
