Petropoulos C J, Yaswen P, Panzica M, Fausto N
Nucleic Acids Res. 1985 Nov 25;13(22):8105-18. doi: 10.1093/nar/13.22.8105.
We examined the methylation pattern and organization of the AFP gene in whole livers and in isolated cell populations purified from livers of rats fed a carcinogenic diet which interferes with DNA methylation. Using restriction endonuclease digestion, we find no differences in methylation pattern and overall organization of the AFP gene in oval cells (AFP-producers) and hepatocytes (non-producers) isolated at the early stages of carcinogenesis. Our studies indicate that in cell populations which produce AFP as well as in cells which are not active in AFP synthesis, the majority of the CCGG sites of the AFP gene are extensively methylated. In addition, we describe the existence of polymorphism in the AFP and albumin genes of Sprague-Dawley rats.
我们研究了喂食干扰DNA甲基化的致癌饮食的大鼠肝脏整体以及从肝脏中分离出的纯化细胞群体中AFP基因的甲基化模式和组织情况。通过限制性内切酶消化，我们发现在致癌早期分离出的卵圆细胞（AFP产生细胞）和肝细胞（非AFP产生细胞）中，AFP基因的甲基化模式和整体组织没有差异。我们的研究表明，在产生AFP的细胞群体以及不活跃于AFP合成的细胞中，AFP基因的大多数CCGG位点都被广泛甲基化。此外，我们描述了Sprague-Dawley大鼠AFP和白蛋白基因中多态性的存在。
