Lucotte G, Gal A, Nahon J L, Sala-Trepat J M
Biochem Genet. 1982 Dec;20(11-12):1105-15. doi: 10.1007/BF00498935.
Two types of variant EcoRI restriction enzyme patterns of albumin-gene DNA fragments have been detected in different rat strains by agarose gel electrophoresis and Southern blot hybridization using 32P-labeled cloned rat albumin cDNA probes. The type I albumin gene variant is characteristic of the Sprague-Dawley strain, and type II is found in Buffalo rats. The occurrence of these variants is interpreted as the result of simple allelic polymorphism because they are inherited in a normal Mendelian fashion when crossing Sprague-Dawley and Buffalo rats. The distribution of the two genetic variants in various inbred strains of rat suggests that type I represents the original or ancestral form of the albumin gene and that type II appeared spontaneously during laboratory breeding.
通过琼脂糖凝胶电泳和使用32P标记的克隆大鼠白蛋白cDNA探针进行的Southern印迹杂交，在不同大鼠品系中检测到了白蛋白基因DNA片段的两种变异型EcoRI限制性酶切图谱。I型白蛋白基因变异是斯普拉格-道利品系的特征，II型则见于布法罗大鼠。这些变异的出现被解释为简单等位基因多态性的结果，因为在斯普拉格-道利大鼠和布法罗大鼠杂交时，它们以正常孟德尔方式遗传。这两种遗传变异在各种大鼠近交系中的分布表明，I型代表白蛋白基因的原始或祖先形式，而II型是在实验室培育过程中自发出现的。
