Hyeon Ji-Yeon, Chon Jung-Whan, Park Jun-Ho, Kim Moo-Sang, Oh Young-Hee, Choi In-Soo, Seo Kun-Ho
College of Veterinary Medicine, Konkuk University, Seoul, Korea . ; Division of Vaccine Research, Korea National Institute of Health, Osong, Korea .
Osong Public Health Res Perspect. 2013 Feb;4(1):27-33. doi: 10.1016/j.phrp.2012.12.005.
To evaluate the abilities of these subtyping methods, we distinguished Salmonella Enteritidis (S. Enteritidis) isolated from food products and human clinical samples between 2009 and 2010 in Seoul using five subtyping methods.
We determined the subtypes of 20 S. Enteritidis isolates from food and human sources using phage typing, antimicrobial susceptibility, pulsed-field gel electrophoresis (PFGE), repetitive sequence-based PCR (rep-PCR), and multi-locus sequence typing (MLST).
A total of 20 tested isolates were differentiated into six antimicrobial susceptibility patterns, three different phage types, four different PFGE profiles, seven rep-PCR patterns, and one MLST type. Food isolates were considerably more susceptible to antibiotics than human isolates. We were best able to discriminate among S. Enteritidis isolates using rep-PCR, and obtained the highest Simpson's diversity index of 0.82, whereas other methods produced indices that were less than 0.71. PFGE pattern appeared to be more related to antimicrobial resistance and phage types of S. Enteritidis isolates than rep-PCR. MLST revealed identical alleles in all isolates at all seven loci examined, indicating no resolution.
The results of this study suggest that rep-PCR provided the best discriminatory power for phenotypically similar S. Enteritidis isolates of food and human origins, whereas the discriminatory ability of MLST may be problematic because of the high sequence conservation of the targeted genes.
为评估这些分型方法的能力，我们使用五种分型方法对2009年至2010年期间在首尔从食品和人类临床样本中分离出的肠炎沙门氏菌（S. Enteritidis）进行区分。
我们使用噬菌体分型、抗菌药敏试验、脉冲场凝胶电泳（PFGE）、基于重复序列的PCR（rep-PCR）和多位点序列分型（MLST）来确定20株来自食品和人类来源的肠炎沙门氏菌分离株的亚型。
总共20株受试分离株被分为六种抗菌药敏模式、三种不同的噬菌体类型、四种不同的PFGE图谱、七种rep-PCR模式和一种MLST类型。食品分离株比人类分离株对抗生素更敏感。我们使用rep-PCR能够最好地区分肠炎沙门氏菌分离株，并获得了最高的辛普森多样性指数0.82，而其他方法产生的指数小于0.71。PFGE图谱似乎比rep-PCR更与肠炎沙门氏菌分离株的抗菌耐药性和噬菌体类型相关。MLST在所有检测的七个位点上在所有分离株中均显示相同的等位基因，表明无分辨能力。
本研究结果表明，rep-PCR对食品和人类来源的表型相似的肠炎沙门氏菌分离株具有最佳的鉴别能力，而由于靶向基因的高序列保守性，MLST的鉴别能力可能存在问题。
