Foley Steven L, White David G, McDermott Patrick F, Walker Robert D, Rhodes Bobbie, Fedorka-Cray Paula J, Simjee Shabbir, Zhao Shaohua
National Farm Medicine Center, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA.
J Clin Microbiol. 2006 Oct;44(10):3569-77. doi: 10.1128/JCM.00745-06.
Molecular characterization (e.g., DNA-based typing methods) of Salmonella isolates is frequently employed to compare and distinguish clinical isolates recovered from animals and from patients with food-borne disease and nosocomial infections. In this study, we compared the abilities of different phenotyping and genotyping methods to distinguish isolates of Salmonella enterica serovar Typhimurium from different food animal sources. One hundred twenty-eight S. enterica serovar Typhimurium strains isolated from cattle, pigs, chickens, and turkeys or derived food products were characterized using pulsed-field gel electrophoresis (PFGE), repetitive element PCR (Rep-PCR), multilocus sequence typing (MLST), plasmid profiling, and antimicrobial susceptibility testing. Among the 128 Salmonella isolates tested, we observed 84 Rep-PCR profiles, 86 PFGE patterns, 89 MLST patterns, 36 plasmid profiles, and 38 susceptibility profiles. The molecular typing methods, i.e., PFGE, MLST, and Rep-PCR, demonstrated the best discriminatory power among Salmonella isolates. However, no apparent correlation was evident between the results of one molecular typing method and those of the others, suggesting that a combination of multiple methods is needed to differentiate S. enterica serovar Typhimurium isolates that genetically cluster according to one particular typing method.
沙门氏菌分离株的分子特征分析（如基于DNA的分型方法）经常用于比较和区分从动物以及食源性疾病和医院感染患者中分离出的临床分离株。在本研究中，我们比较了不同表型分型和基因分型方法区分来自不同食用动物源的肠炎沙门氏菌鼠伤寒血清型分离株的能力。使用脉冲场凝胶电泳（PFGE）、重复元件PCR（Rep-PCR）、多位点序列分型（MLST）、质粒图谱分析和抗菌药敏试验对从牛、猪、鸡和火鸡或其衍生食品中分离出的128株肠炎沙门氏菌鼠伤寒血清型菌株进行了特征分析。在测试的128株沙门氏菌分离株中，我们观察到84种Rep-PCR图谱、86种PFGE模式、89种MLST模式、36种质粒图谱和38种药敏图谱。分子分型方法，即PFGE、MLST和Rep-PCR，在沙门氏菌分离株中显示出最佳的鉴别能力。然而，一种分子分型方法的结果与其他方法的结果之间没有明显的相关性，这表明需要结合多种方法来区分根据一种特定分型方法在基因上聚类的肠炎沙门氏菌鼠伤寒血清型分离株。
