Poonchareon Kritchai, Pulsrikarn Chaiwat, Nuanmuang Narong, Khamai Phichaya
Division of Biochemistry, School of Medical Sciences, University of Phayao, 19 Moo 2, Tambon Maeka, Amphur Muang, Phayao 56000, Thailand.
Department of Medical Sciences, WHO National Salmonella and Shigella Center, National Institute of Health, Ministry of Public Health, Tiwanond Road, Amphur Muang, Nonthaburi 11000, Thailand.
Int J Microbiol. 2019 Jun 17;2019:5086240. doi: 10.1155/2019/5086240. eCollection 2019.
Serotype 4,[5],12:i:-, a monophasic variant of . Typhimurium, with high virulence and multidrug resistance is distributed globally causing pathogenicity to both humans and domesticated animals. BOX-A1R-based repetitive extragenic palindromic-PCR (BOX)-PCR proved to be superior to three other repetitive element-based PCR typing methods, namely, enterobacterial repetitive intergenic consensus (ERIC)-, poly-trinucleotide (GTG)-, and repetitive extragenic palindromic (REP)-PCR (carried out under a single optimized amplification condition), in differentiating genetic relatedness among . 4,[5],12:i:- isolates from feces of hospitalized patients (=12) and isolates from minced pork samples of . 4,[5],12:i:- (=6), . Typhimurium (=6), and Serogroup B (=4) collected from different regions of northern Thailand. Construction of phylogenetic trees from amplicon size patterns allowed allocation of isolates into clusters of similar genetic relatedness, with BOX-PCR generating more unique clusters for each serotype than the other three typing methods. BOX-, (GTG)-, and REP-PCR indicated significant genetic relatedness between . 4,[5],12:i:- isolates 1 and 9 from hospitalized patients and . 4,[5],12:i:- isolate en 29 from minced pork, suggesting a possible route of transmission. Thus, BOX-PCR provides a suitable molecular typing method for discriminating genetic relatedness among spp. of the same and different serotypes and should be suitable for application in typing and tracking route of transmission in outbreaks.
血清型4,[5],12:i:-, 是鼠伤寒沙门氏菌的单相变体，具有高毒力和多重耐药性，在全球范围内分布，对人类和家畜均具有致病性。基于BOX-A1R的重复外源性回文PCR（BOX）-PCR在区分来自住院患者粪便的4,[5],12:i:-分离株（n = 12）以及来自泰国北部不同地区采集的碎猪肉样本中的4,[5],12:i:-（n = 6）、鼠伤寒沙门氏菌（n = 6）和B血清群（n = 4）分离株的遗传相关性方面，被证明优于其他三种基于重复元件的PCR分型方法，即肠杆菌重复基因间共有序列（ERIC）-、聚三核苷酸（GTG）-和重复外源性回文（REP）-PCR（在单一优化扩增条件下进行）。根据扩增片段大小模式构建系统发育树，可将分离株分配到遗传相关性相似的簇中，与其他三种分型方法相比，BOX-PCR为每种血清型产生更多独特的簇。BOX-、（GTG）-和REP-PCR表明，来自住院患者的4,[5],12:i:-分离株1和9与来自碎猪肉的4,[5],12:i:-分离株en 29之间存在显著遗传相关性，提示可能的传播途径。因此，BOX-PCR为区分相同和不同血清型的沙门氏菌属菌株之间的遗传相关性提供了一种合适的分子分型方法，应适用于沙门氏菌属疫情的分型和传播途径追踪。
