a GD - Animal Health Service, Arnsbergstraat 7 , 7418 EZ , Deventer , the Netherlands.
Vet Q. 2013;33(3):132-8. doi: 10.1080/01652176.2013.844378. Epub 2013 Oct 25.
Besides the anticoccidial drug resistance problem, increasing consumer concerns about food safety and residues have propelled the quest for alternative prevention and control strategies amongst which phytotherapy has gained appeal due to a renewed interest in natural medicine.
The objective was in vivo screening of four phytochemicals/extracts and a fungal immunomodulatory protein (FIP) against an Eimeria acervulina infection in broilers.
Four phytochemicals/extracts (extract from Echinacea purpurea, betaine (Betain™), curcumin, carvacrol (two different doses)), and a recombinant FIP from Ganoderma lucidum cloned and expressed in Escherichia coli were investigated for their anticoccidial potential. The experiment was conducted in a battery cage trial with 54 cages of eight birds each. Broilers infected with E. acervulina (a low and high infection dose of 10(4) and 10(5) sporulated oocysts, respectively) and treated with the phytochemicals/extracts or the FIP were compared with broilers treated with the anticoccidial salinomycin sodium (Sacox®) and with an untreated uninfected and an untreated infected control group. Coccidiosis lesion scores, body weight gains and oocyst shedding were used as parameters.
The results showed a coccidiosis infection dose effect on the mean coccidiosis lesion scores. The phytochemicals/extracts and the FIP failed to reduce coccidiosis lesion scores and oocyst shedding, while salinomycin efficiently controlled the E. acervulina infection and enabled significantly higher body weight gains.
In conclusion, the selected phytochemicals/extracts and the FIP did not reduce the lesions of an experimentally induced E. acervulina infection.
除了抗球虫药耐药性问题外，消费者对食品安全和残留问题的日益关注，促使人们寻求替代预防和控制策略，其中植物疗法因其对天然药物的兴趣重新燃起而受到关注。
本研究旨在体内筛选四种植物化学物质/提取物和一种真菌免疫调节蛋白（FIP），以预防鸡柔嫩艾美耳球虫感染。
本研究对四种植物化学物质/提取物（紫锥菊提取物、甜菜碱（Betain™）、姜黄素、香芹酚（两种不同剂量））和一种从灵芝克隆和表达的重组 FIP 进行了抗球虫潜力的研究。该实验采用笼养试验进行，共设 54 个 8 只鸡的笼位。柔嫩艾美耳球虫感染鸡（低感染剂量和高感染剂量分别为 10（4）和 10（5）个孢子化卵囊），用植物化学物质/提取物或 FIP 处理，与用抗球虫药盐霉素钠（Sacox®）处理以及未处理的未感染对照和未处理的感染对照鸡进行比较。用球虫病变评分、体重增加和卵囊脱落作为参数。
结果显示，球虫感染剂量对平均球虫病变评分有影响。植物化学物质/提取物和 FIP 未能降低球虫病变评分和卵囊脱落，而盐霉素能有效控制柔嫩艾美耳球虫感染，使体重增加显著提高。
综上所述，所选植物化学物质/提取物和 FIP 不能减轻实验诱导的柔嫩艾美耳球虫感染的病变。
