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多年生黑麦草细胞质雄性不育（CMS）2. CMS 系的线粒体基因组发生重排，含有嵌合的 atp9 基因。

Cytoplasmic male sterility (CMS) in Lolium perenne L. 2. The mitochondrial genome of a CMS line is rearranged and contains a chimaeric atp 9 gene.

机构信息

Department of Genetics, Trinity College Dublin, 2, Dublin, Ireland.

出版信息

Theor Appl Genet. 1996 Mar;92(3-4):308-15. doi: 10.1007/BF00223673.

DOI:10.1007/BF00223673
PMID:24166251
Abstract

The most striking difference between the mtDNAs of the fertile L. perenne line LPSB21 and the male-sterile line CMS9B290, is the presence in the former and the absence in the latter of a 5.6-kb HindIII fragment. This difference between fertile and sterile lines was the starting point for a detailed molecular analysis of the mitochondrial genome in the region spanning the 5.6-kb HindIII fragment in fertile L. perenne and the corresponding region in CMS9B290. Restriction mapping and Southern-blot analyses indicated that rearrangement of the mitochondrial genome consistent with a deletion/insertion event had occurred in the sterile line. Nucleotide-sequence analysis of the rearranged region in CMS9B290 revealed the presence of (1) a novel chimaeric gene, orf-C9, comprising the first six codons of atp9 fused to a further 118 codons of an unknown sequence and (2) a truncated version of an open reading frame, orf-L, originally identified in LPSB21 mtDNA. Northern-blot analysis confirmed the absence of orf-L transcripts and the presence of orf-C9 transcripts in the mtRNA of CMS9B290.

摘要

最显著的区别在于 mtDNAs 的肥沃 L. perenne 线 LPSB21 和雄性不育线 CMS9B290，是存在的前者和不存在的后者的 5.6-kb HindIII 片段。这种差异之间的肥沃和不育线是出发点的详细的分子分析线粒体基因组跨越 5.6-kb HindIII 片段肥沃的 L. perenne 和相应区域 CMS9B290。限制映射和 Southern-blot 分析表明，重排线粒体基因组一致的删除/插入事件发生在不育线。核苷酸序列分析的重排区域 CMS9B290 揭示了存在（1）一个新的嵌合基因，orf-C9，组成的第一 6 密码子的 atp9 融合到进一步 118 密码子的未知序列和（2）截断版本的开放阅读框，orf-L，最初确定在 LPSB21 mtDNA。Northern-blot 分析证实缺乏 orf-L 转录本和存在的 orf-C9 转录本在 mtRNA CMS9B290。

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本文引用的文献

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Induction of cytoplasmic male-sterility into ryegrass (Lolium perenne).诱导黑麦草（Lolium perenne）细胞质雄性不育。
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多年生黑麦草（Lolium perenne L.）细胞质雄性不育系的线粒体基因组包含一个整合的线性质粒样元件。
Theor Appl Genet. 2008 Aug;117(3):459-70. doi: 10.1007/s00122-008-0790-7. Epub 2008 May 27.
Cytoplasmic male sterility (CMS) in Lolium perenne L.: 1. Development of a diagnostic probe for the male-sterile cytoplasm.
黑麦草细胞质雄性不育（CMS）：1. 不育细胞质诊断探针的开发。
Theor Appl Genet. 1993 Jul;86(6):781-7. doi: 10.1007/BF00222670.
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Paternal inheritance of mitochondria and chloroplasts in Festuca pratensis-Lolium perenne intergeneric hybrids.羊茅-黑麦草属间杂种中线粒体和叶绿体的父系遗传。
Theor Appl Genet. 1994 Jan;87(6):681-8. doi: 10.1007/BF00222893.
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The Texas cytoplasm of maize: cytoplasmic male sterility and disease susceptibility.玉米的得克萨斯细胞质：细胞质雄性不育性和易感性。
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6
Mutation to male fertility and toxin insensitivity in Texas (T)-cytoplasm maize is associated with a frameshift in a mitochondrial open reading frame.德克萨斯州（T）细胞质玉米中的雄性育性和毒素不敏感性突变与线粒体开放阅读框的移码有关。
Proc Natl Acad Sci U S A. 1987 May;84(9):2858-62. doi: 10.1073/pnas.84.9.2858.
7
Nucleotide sequence of F(0)-ATPase proteolipid (subunit 9) gene of maize mitochondria.玉米线粒体 F(0)-ATP 酶脱辅基蛋白（亚基 9）基因的核苷酸序列。
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1015-9. doi: 10.1073/pnas.82.4.1015.
8
A mitochondrial gene is lost via homologous recombination during reversion of CMS T maize to fertility.在线粒体基因通过同源重组丢失，CMS-T 玉米恢复育性的过程中。
EMBO J. 1987 Jun;6(6):1541-6. doi: 10.1002/j.1460-2075.1987.tb02398.x.
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Ogura cytoplasmic male-sterility (CMS)-associated orf138 is translated into a mitochondrial membrane polypeptide in male-sterile Brassica cybrids.小仓细胞质雄性不育（CMS）相关的orf138在雄性不育的芸苔属体细胞杂种中被翻译成一种线粒体膜多肽。
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