Grelon M, Budar F, Bonhomme S, Pelletier G
Laboratoire de Biologie cellulaire, INRA centre de Versailles, France.
Mol Gen Genet. 1994 Jun 3;243(5):540-7. doi: 10.1007/BF00284202.
Transcription of a putative mitochondrial gene (orf138) has previously been correlated with Ogura cytoplasmic male-sterility (CMS) in rapeseed cybrids. In this paper, studies performed on a Brassica cybrid with a different organization of the orf138 locus confirm this association. We also show that mitochondria isolated from male-sterile rapeseed plants synthesize a polypeptide of 19 kDa, which is absent in fertile revertants. Antibodies against a glutathione S-transferase-ORF138 fusion protein were raised to establish that this 19 kDa polypeptide is the product of orf138. The anti-ORF138 serum was used to demonstrate that the orf138 translation product occurs only in sterile cybrids and co-purifies with the mitochondrial membrane fraction.
一个假定的线粒体基因（orf138）的转录先前已与油菜体细胞杂种中的奥古拉面质体雄性不育（CMS）相关联。在本文中，对具有不同orf138基因座组织的芸苔属体细胞杂种进行的研究证实了这种关联。我们还表明，从雄性不育油菜植株中分离出的线粒体合成了一种19 kDa的多肽，而可育回复体中不存在这种多肽。制备了针对谷胱甘肽S-转移酶-ORF138融合蛋白的抗体，以确定这种19 kDa的多肽是orf138的产物。抗ORF138血清用于证明orf138翻译产物仅出现在不育体细胞杂种中，并与线粒体膜部分共纯化。
