随机扩增多态性 DNA（RAPD）标记在啤酒花（Humulus lupulus）中的应用：F1 后代的遗传变异和分离程度。

Random amplified polymorphic DNA (RAPD) markers in hop, Humulus lupulus: level of genetic variability and segregation in F1 progeny.

机构信息

Department of Crop and Soil Sciences, Irrigated Agriculture Research and Extension Center, Washington State University, 99350, Prosser, WA, USA.

出版信息

Theor Appl Genet. 1996 Mar;92(3-4):334-9. doi: 10.1007/BF00223676.

DOI:10.1007/BF00223676

PMID:24166254

Abstract

The level of genetic variation in 24 hop genotypes was studied using the recently developed technique for producing random amplified polymorphic DNAs (RAPDs). Of the 60 primers screened, eight produced polymorphic RAPD bands, 38 produced bands that were monomorphic for all genotypes and 19 did not produce any amplification product. It appeared that the level of polymorphism among the genotypes was generally low. Three of the primers, A11, A17 and C9, were used to determine the stability and segregation of RAPD markers in five families with a total of 182 F1 progeny. The segregation ratios of these markers in the f1 progeny suggested that they were inherited in a Mendelian manner. RAPD markers were stable and may be useful for the construction of linkage maps in hop.

摘要

采用最近发展起来的随机扩增多态性 DNA（RAPD）技术研究了 24 个啤酒花基因型的遗传变异水平。在筛选的 60 个引物中，有 8 个产生多态性 RAPD 带，38 个引物产生所有基因型都相同的单态性带，19 个引物不产生任何扩增产物。似乎基因型之间的多态性水平普遍较低。使用 3 个引物 A11、A17 和 C9 来确定 5 个具有总共 182 个 F1 后代的家系中 RAPD 标记的稳定性和分离。这些标记在 F1 后代中的分离比表明它们是孟德尔遗传的。RAPD 标记是稳定的，可能对啤酒花的连锁图谱的构建有用。