RAPD 变异的定性和定量描述在菜豆（菜豆）基因型之间。

Qualitative and quantitative characterization of RAPD variation among snap bean (Phaseolus vulgaris) genotypes.

机构信息

Department of Horticulture, University of Wisconsin, 53706, Madison, Wisconsin, USA.

出版信息

Theor Appl Genet. 1995 Nov;91(6-7):1078-85. doi: 10.1007/BF00223922.

Abstract

Ten snap bean (Phaseolus vulgaris) genotypes were screened for polymorphism with 400 RAPD (random amplified polymorphic DNA) primers. Polymorphic RAPDs were scored and classified into three categories based on ethidium bromide staining intensity. An average of 5.19 RAPD bands were scored per primer for the 364 primers that gave scorable amplification products. An average of 2.15 polymorphic RAPDs were detected per primer. The results show that primer screening may reduce the number of RAPD reactions required for the analysis of genetic relationships among snap-bean genotypes by over 60%. Based on the analysis of the distribution of RAPD amplification, the same number of polymorphic RAPDs were amplified from different genotypes for all RAPD band intensity levels. A comparison of RAPD band amplification frequency among genotypes for the three categories of bands classified by amplification strength revealed a measurable difference in the frequencies of RAPDs classified as faint (weakly amplifying) compared to RAPD bands classified as bold (strongly amplifying) indicating a possible scoring error due to the underscoring of faint bands. Correlation analysis showed that RAPD bands amplified by the same primer are not more closely correlated then RAPD bands amplified by different primers but are more highly correlated then expected by chance. Pairwise comparisons of RAPD bands indicate that the distribution of RAPD amplification among genotypes will be a useful criterion for establishing RAPD band identity. For the average pairwise comparison of genotypes, 50% of primers tested and 15.8% of all scored RAPDs detected polymorphism. Based on RAPD data Nei's average gene diversity at a locus was 0.158 based on all scorable RAPD bands and 0.388 if only polymorphic RAPD loci were considered. RAPD-derived 1 relationships among genotypes are reported for the ten genotypes included in this study. The data presented here demonstrate that many informative, polymorphic RAPDs can be found among snap bean cultivars. These RAPDs may be useful for the unique identification of bean varieties, the organization of bean germplasm, and applications of molecular markers to bean breeding.

摘要

对 10 个菜豆（Phaseolus vulgaris）基因型进行了 400 个随机扩增多态性 DNA（RAPD）引物的多态性筛选。根据溴化乙锭染色强度，将扩增产物分为 3 类，并对多态性 RAPD 进行评分。在可评分的 364 个扩增产物中，每个引物平均可获得 5.19 个 RAPD 条带。每个引物平均可检测到 2.15 个多态性 RAPD。结果表明，通过引物筛选，可将分析菜豆基因型遗传关系所需的 RAPD 反应数减少 60%以上。根据 RAPD 扩增分布的分析，在所有 RAPD 带强度水平下，不同基因型都可扩增出相同数量的多态性 RAPD。对按扩增强度分类的 3 类 RAPD 带的基因型间 RAPD 带扩增频率的比较表明，与被归类为强（强扩增）的 RAPD 带相比，被归类为弱（弱扩增）的 RAPD 带的扩增频率有明显差异，这可能是由于对弱带的低估导致的评分误差。相关性分析表明，由同一引物扩增的 RAPD 带并不比由不同引物扩增的 RAPD 带更密切相关，但比随机预期的相关性更高。对 RAPD 带的成对比较表明，基因型间 RAPD 扩增的分布将是建立 RAPD 带同一性的有用标准。对于平均的基因型成对比较，测试的 50%的引物和 15.8%的所有评分 RAPD 检测到多态性。基于 RAPD 数据，根据所有可评分的 RAPD 带，每个位点的 Nei 平均基因多样性为 0.158；如果只考虑多态性 RAPD 位点，则为 0.388。报道了包括在本研究中的 10 个基因型之间的 RAPD 衍生 1 关系。本文提供的数据表明，在菜豆品种中可以发现许多信息丰富的多态性 RAPD。这些 RAPD 可能对豆科品种的独特鉴定、豆科种质的组织和分子标记在豆类育种中的应用有用。