Department of Molecular Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, AA Wageningen, The Netherlands.
Theor Appl Genet. 1996 Mar;92(3-4):463-70. doi: 10.1007/BF00223694.
A backcross population, derived from the cross (S. tuberosumxS. spegazzinii)xS. tuberosum was used to map QTLs involved in nematode resistance, tuber yield and root development. Complete linkage maps were available for the interspecific hybrid parent as well as the S. tuberosum parent, and interval mapping for all traits was performed for both. Additionally, the intra- and inter-locus interactions of the QTLs were examined. The Gro1.2 locus, involved in resistance to G. rostochiensis pathotype Ro1, that was previously mapped in the S. tuberosumxS. spegazzinii F1 population, was located more precisely on chromosome 10. A new resistance locus, Gro1.4, also conferring resistance to G. rostochiensis pathotype Ro1, was found on chromosome 3. Different alleles of this locus originating from both parents contributed to the resistant phenotype, indicating multiallelism at this locus. No interlocus interactions were observed between these two resistance loci. For resistance to G. pallida no QTLs were detected. One minor QTL involved in tuber yield was located on chromosome 4. Two QTLs involved in root development and having large effects were mapped on chromosomes 2 and 6 and an epistatic interaction was found between these two loci.
利用源自杂交（Solanum tuberosum×S. spegazzinii）×S. tuberosum 的回交群体，对与线虫抗性、块茎产量和根系发育相关的 QTL 进行了作图。杂种亲本和 S. tuberosum 亲本均具有完整的连锁图谱，对所有性状进行了区间作图。此外，还检查了 QTL 的等位基因内和等位基因间相互作用。Gro1.2 位点，先前在 S. tuberosum×S. spegazzinii F1 群体中映射到 10 号染色体，与抗根结线虫 G. rostochiensis 致病型 Ro1 有关，定位更加精确。在 3 号染色体上发现了一个新的抗性位点 Gro1.4，也能抗 G. rostochiensis 致病型 Ro1。这个位点来自双亲的不同等位基因有助于抗性表型，表明该位点的多等位性。这两个抗性位点之间没有观察到基因间相互作用。对 G. pallida 的抗性没有检测到 QTL。一个与块茎产量有关的较小 QTL 位于 4 号染色体上。两个与根系发育有关且具有较大效应的 QTL 被定位在 2 号和 6 号染色体上，并发现这两个位点之间存在上位性相互作用。
